拟南芥DNA_J结构域蛋白的生物信息学分析及其亚细胞定位验证  

Bioinformatics and subcellular localization analysis of a protein containing the DNA_J domain of Arabidopsis thaliana

作　　者：宁正元[1] 王爱荣[1] 林文伟[2] 林德书[2] 张冬梅[2] 刘泓[2] 周洁[2] 王宗华[2] 

机构地区：[1]福建农林大学计算机与信息学院 [2]福建农林大学功能基因组学研究中心,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2007年第4期427-434,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(D0510020);国家自然科学基金资助项目(30471178)

摘　　要：对拟南芥RSS1蛋白进行了一系列生物信息学分析,结果表明:该蛋白是一C端含有DNA_J结构域的辅助蛋白,其N端含有受体蛋白复合物AP-2结合区DPF/W和FxDxF区,中间区域含有可信度较低的丝氨酸富集区和谷氨酸富集区.该蛋白可能具有ATP结合活性、丝氨酸/苏氨酸激酶活性和磷酸酶活性,可调控细胞循环和蛋白的磷酸化和去磷酸化作用.利用洋葱表皮瞬时表达系统验证了RSS1-GFP融合蛋白定位于细胞核中,为进一步研究该蛋白的分子和生物学功能奠定了良好基础.A series of bioinformatics analysis of RSS1 protein ofArabidopsis thaliana was analyzed. The result showed that RSS1 had a J-domain, resembling clathrin-uncoating factor auxilin at its C terminus. It also contained an Asp-Pro-Phe （DPF） adaptor proteinbinding motif and FxDxF motif at the N terminus, which was known to bind to the appendage domain of α or β subunits of an adaptor protein complex AP-2. Moreover, there were a serine-rich region and a glutamic acid-rich region in RSS1. The bioinformatics data suggested that RSS1 might have ATP-binding activity, serine/thrine kinase or phosphatase activity and it might regulate cell cycle or phosphorylation or dephosphorylation of proteins. Subceilular localization by instant expression of RSS1-GFP fusion protein on onion epidermis showed that RSS1 was localized in nucleolus, which would help to further characterize molecular or biological func- tions of the protein.

关 键 词：拟南芥 辅助蛋白 J结构域 生物信息学 

分 类 号：Q7[生物学—分子生物学]