利用中国明对虾单对杂交亲本及其F_2群体构建RAPD遗传连锁图谱  

RAPD linkage map of Chinese shrimp(Fenneropenaeus chinensis) constructed using one pair of parents and the F_2 progeny as segregating population

作　　者：李晓静[1] 王伟继[1] 孔杰[1] 孟宪红[1] 田燚[1] 孟鹏[1] 毛连菊[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]大连水产学院,辽宁大连116023

出　　处：《中国水产科学》2007年第5期770-777,共8页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(30500378);农业部948引进项目(2006-G55(B))

摘　　要：由中国明对虾(Fenneropenaeus chinensis)单对杂交亲本(G♀和G♂)及其F2为作图群体,构建了中国明对虾RAPD分子标记的遗传连锁图谱。109个引物共产生284个符合孟德尔分离规律的位点,符合1∶1孟德尔分离类型的位点共234个,符合3∶1孟德尔分离类型的位点50个。利用拟测交理论分别构建中国明对虾雌虾、雄虾的遗传连锁图谱。107个1∶1分离标记分布于雌虾连锁图谱中,包括31个连锁群,图谱总长度为1 406.1 cM,所有标记间的平均间隔为18.5 cM;91个1∶1分离标记分布于雄虾连锁图谱中,包括26个连锁群,图谱总长度为1 187.7 cM,所有标记间的平均间隔为18.27 cM;利用F2自交模型构建了雌虾和雄虾共同的分子标记,35个3∶1分离标记分布于雌雄共有的连锁图谱中,包括10个连锁群,图谱总长度为432.9 cM,所有标记间的平均间隔为17.3 cM。RAPD linkage maps of Fenneropenaeus chinensis were constructed using one pair of parent and the F2 progeny （full sib family） as segregating population. 109 primers produced 284 bands yielding Mendelian segregation, in which 234 bands segregated in 1 ： 1 model and 50 in 3 ： 1 model. The 1 ： 1 ratio markers from female and male were used to construct their linkage maps respectively based on the pseudotestcross strategy. The female linkage maps of F. chinensis containing 107 segregating markers consisted of 31 linkage groups （including linkage couples） covering 1 406.1 cM with the average interval of 18.5 cM, and the male linkage maps containing 91 segregating markers linked in 25 linkage groups covering 1 187.7 cM with the average interval of 18.27 cM. The 36 markers in 3 ： 1 model were used to construct the common linkage maps of F. chinensis based on F2 intercross model strategy. The common linkage maps consist of 10 linkage groups with 36 markers, covering 432.9 cM of F. chinensis genome with the average interval of 17.3 cM. [ Journal of Fishery Sciences of China, 2007,14 （5） ： 770 - 777 ]

关 键 词：中国明对虾 RAPD分子标记 遗传连锁图谱 

分 类 号：S96[农业科学—水产养殖]