大亚湾海域春季浮游植物生长的限制性营养元素  

Nutrient factors limiting phytoplankton production of Daya Bay in spring

作　　者：王晓伟[1] 李纯厚[1] 李占东[1] 林琳[1] 沈南南[1] 

机构地区：[1]中国水产科学研究院南海水产研究所农业部渔业生态环境重点开放实验室

出　　处：《中国水产科学》2007年第5期836-842,共7页Journal of Fishery Sciences of China

基　　金：科技部科研院所社会公益研究专项(2005DIB3J020)

摘　　要：2006年4月,模拟现场条件对大亚湾浮游植物生长限制性营养素进行了初步研究。整个海湾设置3个站点(S1,S2,S3),每个站点设计7组实验,实验周期3天。通过比较元素耗尽的先后顺序和显著性差异评估限制性营养元素。两种方法具有一定的互补性。湾中部(S2)和湾顶(S3)站水样添加的磷元素在48 h内被耗尽,最大吸收速率达到0.83μmol/(L.h),先于其他两种元素而被耗尽,显示出明显的磷限制;湾口(S1)站氮元素的吸收速率高于其他各元素,最大达到0.76μmol/(L.h),94 h内耗尽,而磷和硅则分别需要107 h和114 h,但是由于磷偏离Redfield比值10倍于氮、硅添加,所以氮限制的结果并不确定。显著性分析结果显示,S1各添加氮的实验组生物量都有显著性增加,氮限制是十分明显,S2站叶绿素a浓度差异性结果不明显。S3站叶绿素a的差异显著性结果与营养元素耗尽评估结果一致。两种方法研究结果表明,大亚湾海域浮游植物限制性元素发生了转变,湾中和湾顶为磷限制,湾口为氮限制,大亚湾海域流场的区域差异可能是造成这种现象的主要原因。Daya Bay, a rapidly urbanizing area, received anthropogenic nutrient loading. Some researches showed the primary limitation nutrient in Daya Bay was experiencing the transition from N-limit to P-limit. Changes in nutrient regime could have negative ecological effects on the bay. Phytoplankton nutrient limitation was studied during spring （April 2006）. Seven factorial nutrient enrichment experiments were carried out in various locations in the bay, including outer middle inter of the bay, The experiments included additions of nitrogen （N）, phosphorus （P） and silicate （Si） at a mole ratio of 16N ： 10P： 16Si, additions of iron （Fe） and a synthetic chelator ethylenediaminetetraacetic acid （EDTA）. The water was sampled from 3 stations and the experiment period was 3 days. To evaluate the nutrient limitation patterns, time series of chlorophyll a concentrations were analyzed using one-way ANOVA model and ranking method and the apparent nutrients depletion time were estimated. The two ways could explain each other. According to depletion time sequence results, phosphorus was running out in 48 h at St. 3 and St. 2, revealing that phytoplankton was limited by phosphorus at St. 2 and St. 3. At St. 1, nitrogen depletion time （94 h）was faster than the others （P depletion time 107 h and Si depletion time 114 h）. As the phosphorus deviation the Redfield ratio by 10 times,the result showed that N-limitation was not so determinate. For the analysis results of chlorophyll a concentrations, St. 1 was N-limitation obviously. For St. 2, the chlorophyll a analysis results showed P and Si co-limited. Compared with the depletion time sequence, because each nutrient had a high bioavailability, the phytoplankton grew well in every experiment unit at the station. As for chlorophyll a concentration, the variance between experiment units and control unit was not so significant. The analysis results of chlorophyll a concentrations at St. 3 accorded with that of depletion time sequence. Iron had a positive

关 键 词：大亚湾 浮游植物 营养盐限制 加富实验 

分 类 号：S922.9[农业科学—渔业资源]