牛带绦虫亚洲亚种核糖体蛋白L10a基因分析  

Informatics Analysis of 60S Ribosomal Protein L10a Gene of Taenia asiatica

作　　者：申萍香[1] 黄江[1] 周灵贵[1] 廖兴江[1] 郎书源[2] 

机构地区：[1]贵阳医学院多媒体形态学实验室,贵州贵阳550004 [2]贵阳医学院寄生虫学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2007年第4期365-367,共3页Journal of Guiyang Medical College

基　　金：贵州省科技攻关项目[黔科合NY字(2006)3037]

摘　　要：目的：分析和预测牛带绦虫亚洲亚种成虫核糖体蛋白LlOa基因及其编码蛋白的结构和功能。方法：构建牛带绦虫亚洲亚种成虫eDNA文库，进行EST测序，将EST序列与GenBank中登陆的序列进行同源性比对及基因完整性判断；应用NCBI上的BLAST对所筛选基因的保守域进行搜索比对，利用PredictProtein分析、预测其功能域及二级结构。结果：BLASTX分析该基因为全长基因，全长698bp，编码区为24-681，编码218个氨基酸，无跨膜区，具有较稳定的理化性质。结论：从牛带绦虫亚洲亚种成虫cDNA文库中筛选出核糖体蛋白L10a基因。Objective： To analyze and predict the constructions and functions of 60S ribosomal protein L10a gene of Taenia asiatica and its protein product. Methods： Constructing a cDNA library of a- dult Taenia asiaticas, sequencing a large quantity of expressed sequence tags （ EST）, comparing the sequences of EST with those reported in GenBank for analysis of homology, then, performing sequence splicing and analyzing gene integrity with RPSBLAST program and predicting its functional domain and secondary structure with PredictProtein program. Results： The results showed that the gene is 698bp in length and its coding region is 24 -681, coding 218 amino acids. It is a complete fuU-length gene when compared with the homologues in C, enBank. The coded protein has stable phycical-chemical characteristics, but dose not have transmembrane regions. Conclusion：60S ribosomal protein L10a gene was screened from the cDNA library of adult Taenia asiaticas by using bioinformatical method.

关 键 词：无钩绦虫牛肉绦虫 核糖体蛋白质类 基因顺序 

分 类 号：R383.32[医药卫生—医学寄生虫学]