反义核酸对人结肠癌SW480细胞存活素表达和增殖的抑制  

The Inhibitory Effect of Antisense Oligonucleotide Targeting Survivin to the Proliferation of SW480 Cells

作　　者：潘克俭[1] 杨平[1] 王玉明[1] 王西岩[2] 

机构地区：[1]成都医学院存活素生物化学教研室 [2]成都医学院临床技能中心,四川成都610083

出　　处：《贵阳医学院学报》2007年第4期368-370,374,共4页Journal of Guiyang Medical College

基　　金：四川省应用基础重点项目(2006J13-022)

摘　　要：目的:研究存活素(Survivin)反义寡核苷酸对人结肠癌SW480细胞增殖的影响。方法:针对Sur-vivin mRNA序列设计了反义寡核甘酸,转染SW480细胞;RT-PCR检测细胞中Survivin基因的mRNA的表达,Western blot显示Survivin蛋白水平,以MTT法检测细胞增殖。结果:Survivin反义寡核苷酸可明显降低SW480细胞中Survivin的mRNA和蛋白水平;MTT比色法结果说明人Survivin反义寡核苷酸抑制SW480细胞增殖,抑制率远高于反义寡核苷酸组和空白对照组。结论:Survivin反义寡核苷酸能有效的下调SW480细胞Survivin的表达,同时抑制细胞增殖,有望应用于人结肠癌辅助治疗之中。Objective： To investigate the effect of survivin antisense oligonucleotide （ASODN） to the proliferation of SW480 cells. Mathods： A human ASODN was designed, synthesized, and transfected into SW480 cells. Expression of survivin in SW480 cells was detedted with RT-PCR and westem blot. The inhibition of cell proliferation by ASODN was demonstrated with MTI＇ assay. A nonsense oligonu- cleotide fragment （SODN） was designed as one control. Results： The expression of survivin mRNA and protein in ASODN treated group was significantly lower than that in control groups. Conclusion： ASODN can knock-down survivin expression in SW480 cells and inhibit cell proliferation. It′s promis- ing to use ASODN in adjunctive theraov of human colon carcinoma.

关 键 词：寡核苷酸类 反义 癌 结肠肿瘤 存活素 

分 类 号：R342.4[医药卫生—基础医学] R735.35