子痫前期胎盘组织差异表达基因ABCG_2的克隆和鉴定  

Differential Expression of ABCG_2 Gene in Placentas of Preeclampsia

作　　者：贾艳菊[1] 陈叙[1] 刘映粦[2] 

机构地区：[1]天津市中心妇产科医院,天津300052 [2]天津市医科大学第二医院,天津300052

出　　处：《生殖与避孕》2007年第8期514-517,540,共5页Reproduction and Contraception

基　　金：天津市自然科学基金项目;项目号:033610011

摘　　要：目的:克隆和鉴定子痫前期胎盘组织差异表达基因,确定与子痫前期相关的基因。方法:应用荧光mRNA差异显示技术寻找并克隆子痫前期胎盘组织差异表达的基因,对其中的ATP结合盒转运子G_2(ATP binding cassette transporter G_2,ABCG_2)基因用半定量RT-PCR技术验证其表达。结果:ABCG_2基因在子痫前期胎盘组织中高表达,半定量RT-PCR结果与mRNA差异显示的结果相符合,ABCG_2基因在子痫前期胎盘组织中的表达高于正常胎盘组织,差异有显著性(P<0.05)。结论:荧光mRNA差异显示技术可用于筛选子痫前期胎盘组织差异表达基因,并应用此技术发现了ABCG_2基因在子痫前期患者胎盘组织表达水平增高。Objective： To clone and identify the genes differentially expressed in placentas of preedampsia and determine which involved in pathogenesis ofpreeclampsia. Methods： Fluorescent differential display （FDD） was used to find and clone the differentially expressed genes in placentas ofpreeclampsia, the expression of the ATP binding cassette transporter G2 （ABCG2） gene was verified by semi-quantitive reverse transcription- polymerase chain reaction（RT-PCR）. Results： The ABCG2 gene was found by FDD and its expression was up-regulated in placentas ofpreeclampsia, it was confirmed by RT-PCR that the rnRNA expression of ABCG2 gene was significantly higher in placentas ofpreeclampsia than that in the matched ones（P 〈 0.05）. Conclusion： FDD can be used for screening differentially expressed genes in placentas of preeclampsia.

关 键 词：子痫前期 胎盘 差异显示 RT—PCR ABCG2 

分 类 号：R714.2[医药卫生—妇产科学]