一氧化氮在乙烯诱导蚕豆气孔关闭中的作用  

The Role of Nitric Oxide in Ethylene-induced Stomatal Closure in Vicia faba L.

作　　者：李杰[1] 邱丽艳[1] 赵方贵[1] 侯丽霞[1] 刘新[1] 

机构地区：[1]青岛农业大学生命科学学院

出　　处：《植物生理与分子生物学学报》2007年第4期349-353,共5页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金(No.30370141);山东省教育厅基金(No.J04C13)项目资助~~

摘　　要：以蚕豆为材料研究了一氧化氮(nitric oxide,NO)和乙烯对气孔运动的影响。结果表明,10μmol/L的NO供体硝普钠(sodium nitroprusside,SNP)以及0.04%的乙烯能明显诱导蚕豆气孔关闭,并且二者共同处理后,能够增强其促进气孔关闭的作用。乙烯合成抑制剂AVG可以减弱NO诱导气孔关闭的程度,NO清除剂c-PTIO和NR抑制剂NaN3也可减弱乙烯诱导气孔关闭的程度,而一氧化氮合酶(nitric oxide synthase,NOS)抑制剂L-NAME对乙烯诱导气孔关闭的作用不明显。推测,在调控蚕豆气孔关闭过程中,NO可能主要通过NR途径参与乙烯调控气孔关闭过程。The effects of nitric oxide （NO） and ethylene on Vicia faba L. stomatal movement were studied. The results showed that NO donor SNP （sodium nitroprusside） 10 μmol/L and ethylene 0.04% could induce stomatal closure distinctly and they could promote stomatal closure when treated together （Figs. 1-3）. When treated with AVG （an inhibitor of ethylene synthesis）, c-PTIO （a specific scavenger of NO） and NaN3 （an inhibitor of NR）, the effects of NO-and ethylene-induced stomatal closure were inhibited but the inhibitor of nitric oxide synthase （NOS） had little effect （Figs.4, 5）.We presumed that there was coordinative effect between NO and ethylene in regulation of stomatal closure; ethylene could induce stomatal closure by regulating the production of nitrate reductase （NR）- dependent NO.

关 键 词：气孔运动 一氧化氮 乙烯 蚕豆 

分 类 号：Q945[生物学—植物学]