0.7MPa(7ATA)高气压下大鼠脊髓突触体甘氨酸摄取的变化  

CHANGES OF GLYCINE UPTAKE OF SYNAPTOSOMES OF RAT SPINAL CORD IN 0.7MPa(7ATA) AIR PRESSURE

作　　者：毕钢[1] 张剑[1] 杨涛[1] 刘景昌[1] 

机构地区：[1]海军医学研究所潜水医学研究室,上海200433

出　　处：《中国应用生理学杂志》2007年第3期334-337,共4页Chinese Journal of Applied Physiology

基　　金：全军医药卫生科研基金课题(98M049)

摘　　要：目的:研究氮麻醉时甘氨酸神经递质功能变化。方法:制备脊髓突触体,用同位素方法观察0.7MPa(7ATA)高压空气环境中大鼠脊髓突触体摄取甘氨酸的情况。结果:0.7MPa(7ATA)时甘氨酸摄取速度减慢,达到饱和摄取量时间延长,最大饱和摄取量下降。甘氨酸摄取的Vm减小,Km增加。在加入10-7mol.L-1皮质酮后,可增加0.7MPa(7ATA)高压空气时甘氨酸摄取Vm。结论:在高气压下发生氮麻醉时,甘氨酸摄取转运体的功能降低,与甘氨酸亲和力下降;皮质酮有助于高亲和力甘氨酸转运体功能恢复。Aim：To study changes of function of transmitter glycine in nitrogen narcosis.Methods：Synaptosomes of rat spinal cord were prepared.Glycine uptake of synaptosomes of rat spinal cord in 0.7 MPa（7ATA）hyperbaric air pressure was observed by the methods of isotope.Results：Glycine uptake slowed down and took a longer period of time to reach saturation in 0.7 MPa（7ATA）.The maximum glycine uptake was lessened.Vm was diminished,but Km was increased.Vm rose in 0.7 MPa（7ATA）when corticosterone was added.Conclusion：When nitrogen narcosis arose in 0.7 MPa（7ATA）,the function of transporters of glycine re-uptake was reduced,the affinity of glycine for transporters subsided.Corticosterone was conductive to the recovery of the function of glycine transporters of high affinity.

关 键 词：氮麻醉 甘氨酸 摄取 甘氨酸转运体 

分 类 号：R122.2[医药卫生—环境卫生学] R338.7[医药卫生—公共卫生与预防医学]