核因子-κB在烧伤血清复合缺氧致心肌细胞损伤中的作用  

NF-κB imvolved in the process of cardiomyocytes damage caused by burn sera and hypoxia

作　　者：范鹏举[1] 李珍[2] 黄晓元[1] 郑军[3] 黄跃生[4] 雷少荣[1] 

机构地区：[1]中南大学湘雅医院烧伤整形科,长沙410008 [2]中南大学湘雅医院中心ICU,长沙410008 [3]南华大学附一院烧伤科 [4]第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室

出　　处：《中华急诊医学杂志》2007年第8期829-832,共4页Chinese Journal of Emergency Medicine

基　　金：国家重点基础研究发展规划资助项目（“973”项目）（G1999054205）;国家杰出青年科学基金资助项目（30125040）

摘　　要：目的探讨NF-κB在烧伤血清复合缺氧致心肌细胞损伤中的作用。方法体外培养SD大鼠乳鼠心肌细胞,按施加刺激因素及是否进行拮抗剂预处理分为四组：正常对照组（n=4）;烧伤缺氧组（n=4）,10%自制大鼠烧伤血清＋缺氧（1%氧浓度）刺激6h;PDTC组（n=4）,于培养基中加入NF-κB特异抑制剂吡咯烷二硫代氨基甲酸盐（PDTC,50mmol/L）;PDTC预处理组（n=4）,PDTC预处理30min后,施加烧伤缺氧刺激;MTT法检测心肌细胞活力;提取核蛋白,蛋白免疫印迹化学发光法检测NF-κB核内含量;EMSA检测NF-κBDNA结合活性。结果烧伤缺氧组NF-κB核内水平较正常组显著升高,DNA结合活性提高,心肌细胞活力下降;PDTC预处理后,核内NF-κB水平较烧伤缺氧组明显降低,DNA结合活性显著降低,烧伤缺氧所致的心肌细胞损伤明显减轻,活力提高。结论NF-κB参与烧伤缺氧致心肌细胞损伤作用,阻断NF-κB可能对防治严重烧伤所致的心肌细胞损伤具有一定潜在价值。Objectove To explore the role of NF-κB in the cardiomyocyte＇ s amage by burn sera and hypoxia. Method Ventricular cardiomyocytes isolated from neonatal rats were used in this study. Rats were randomly divided into four groups： control group （ n = 4）, burn and hypoxia group simulated by 10% self-made rat burn serum ＋ 6 hours of hypoxia （ 1% O2 ） （ n = 4）, PDTC group by adding PDTC （ pyrrolidine dithiocarbamate） to the medium （ n = 4）, and PDTC pretreating group pretreated by PDTC for 30 min and then treated with burn serum ＋ hypoxia for 6 hours （ n = 4）. The activity of cardiomyocytes was detected by MTI＇. The content of NF-κB in the nuclear was detected by west blotting and its DNA binding activity was detected by EMSA. Results The level of NF-κB-p50 in the nuclear raised markedly after burn serum ＋ hypoxia exposure, and its DNA binding activity was elevated remarkably, but the viability of cardiomyocytes decreased. After PDTC pretreating, the content of NF-κB-p50 in the nuclear decreased compared to burn serum and hypoxia group, and its DNA binding activity decreased. The damage of cardiomyocytes was significantly lessened, and the vigor of cardiomyocytes was improved. Conclusions NF-κB is involved in the procedure of cardiomyocytes damage caused by burn serum and hypoxia exposure. It may benefit the cardiomyocytes by blocking NF-κB in severe burn patients.

关 键 词：缺氧 烧伤 核因子-ΚB 

分 类 号：R686[医药卫生—骨科学]