Me_2SO浓度及预处理条件对组织工程化真皮活性的影响  

Effects of Me_2SO and loading conditions on the cell viability of tissue -engineered dermal replacement

作　　者：王欣[1] 华泽钊[1] 刘宝林[1] 杨光辉 崔磊 曹谊林 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]国家组织工程研究与开发中心,上海200235

出　　处：《上海理工大学学报》2007年第4期311-314,共4页Journal of University of Shanghai For Science and Technology

基　　金：国家自然科学基金重点资助项目(50436030);国家自然科学基金资助项目(50376040);上海市重点学科建设资助项目(T0503);上海市教委发展基金资助项目(F50119)

摘　　要：以二甲基亚砜（Me2SO）为低温保护剂,在就Me2SO浓度、处理温度及时间对真皮成纤维细胞的活性影响进行研究的基础上,研究了Me2SO浓度对低温保存后组织工程化真皮活性的影响.采用胎盼蓝拒染法和四唑盐（MTT）比色法检测细胞存活率.结果表明,Me2SO浓度、预处理时间及温度对细胞及组织的活性有明显影响.将添加低温保护剂后的细胞悬液在4℃下保持20 min有利于保持较高的细胞活性;且当Me2SO的体积分数在10%-20%时,可使低温保存后的组织工程化真皮组织的细胞活性保持在60%以上.In order to optimize the cryopreservation protocols of tissue engineered dermal equivalent, the effects of cryoprotectant concentration and the loading conditions on the cell viability of tissue-engineered dermal replacement were studied with dimethyl sulfoxide（Me2SO） as the cryoprotectant. The effects of Me2SO concentration and the exposure time/temperature to Me2SO on the cell viability of dermal fibroblasts were studied. Then experiment was conducted to investigate the effect of Me2SO concentration on the cell viability of freeze-thawed tissue-engineered dermal replacement, under the selected pretreatment conditions. Viabilities of the fibroblast and the dermal slices were determined by using the Trypan Blue staining and the modified MTT assay, respectively. The results indicate that the cryoprotectant concentration （Me2SO） and the loading conditions （pretreatment time and tempera- ture） have significant effects on the cell viability of the dermal fibroblast and the tissue-engineered dermal equivalent. In order to maintain a relatively higher cell viability（≥60 % ） during： the cryoprotectant loading stage, a combination of 4 ℃ and 20 min is preferable. And if the Me2SO concentration ranged from 10% to 20% ,the freeze-thawed dermal equivalent can retain the cell viability over 60%.

关 键 词：二甲基亚砜 组织工程 真皮替代物 低温保存 

分 类 号：R318.52[医药卫生—生物医学工程]