杨树湿地松组织培养愈伤组织耐盐性  

Salt tolerance of calli of Populus clones and Pinus elliottii in tissue cultures

作　　者：张立钦[1] 郑勇平[2] 罗士元 胡加共 

机构地区：[1]浙江林学院林学系 [2]浙江省林业厅种苗站 [3]浙江省台州地区林业特产局

出　　处：《浙江林学院学报》1997年第1期16-21,共6页Journal of Zhejiang Forestry College

基　　金：浙江省自然科学基金

摘　　要：对杨树无性系ＮＬ－８０１０５，ＮＬ－８０１０６，ＮＬ－８０１１７和湿地松组织培养愈伤组织的耐盐性进行了研究。结果表明：以ＭＳ为基本培养基，添加２，４－Ｄ２．０ｍｇ·ｋｇ－１和ＫＴ０．５ｍｇ·ｋｇ－１，诱导这些树种愈伤组织效果好；在含氯化钠的培养基上，杨树不同器官外植体的愈伤组织诱导能力和耐盐程度有差异，外植体茎、叶比根强；杨树继代愈伤组织的耐盐能力显著高于湿地松，这与植株水平的耐盐性相一致。The tolerance to NaCl of tissues culture calli of Populus clones(NL 80105,NL 80106,and NL 80117) and Pinus elliottii was studied The results were as follows:(1) MS medium with 2 0 mg·kg -1 of 2,4 D and 0 5 mg·kg -1 KT was suitable for tree calli in tissue cultures;(2) the salt tolerances of calli from roots,stems and petioles were different on MS containing NaCl The calli with the highest salt tolerance was from stems;(3)the salt tolerance of subculture calli of Populus clones was higher than that of Pinus elliottii,which was in keeping with the salt tolerance of  Populus and Pinus elliottii trees

关 键 词：杨属 湿地松 组织培养 愈伤组织 耐盐性 

分 类 号：S722.36[农业科学—林木遗传育种]