薯蓣皂苷水解微生物的筛选  

Selection of the Microorganism for Dioscin Hydrolyze

作　　者：赵玉婷[1] 徐增莱[2] 戴传超[1] 汤兴利[2] 夏冰[2] 汪琼[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]江苏省中科院植物研究所/南京中山植物园,江苏南京210014

出　　处：《中药材》2007年第8期905-909,共5页Journal of Chinese Medicinal Materials

基　　金：江苏省药用植物生物技术重点实验室开放项目(KJS03079)

摘　　要：目的:筛选可水解薯蓣皂苷的微生物。方法:从盾叶薯蓣根茎表面、根际土壤、叶片及根茎内筛选可降解薯蓣皂苷得皂苷元的菌株,薯蓣皂苷元采用薄层层析和高效液相色谱法鉴定。结果:筛选得到水解薯蓣皂苷的微生物,经鉴定分别属于曲霉属和交链孢属,并对其中一株菌的产酶特性和发酵工艺进行了初步研究。结论:筛选菌株分泌的薯蓣皂苷水解酶,可水解薯蓣皂苷得到皂苷元。Objective： To select the microorganism which can hydrolyze dioscin to diosgenin. Methods： The microorganism were selected from the surface of rhizome, rhizosphere soil, the inside of the leaves and rhizome of Dioscorea zingiberensis C. H. Wright.Diosgenin was identified by thin-layer chromatography and HPLC. Results： The microorganism which could hydrolyze dioscin from the experiment were identified as Aspergillus sp and Alternaria sp. Characteristics of enzymes production and fermentation technology of Aspergillus No. 1 were also studied primarily. Conclusion： The Aspergillus strain No. 1 can secret enzyme to hydrolyze dioscin into diosgenin effectively.

关 键 词：薯蓣皂苷 薯蓣皂苷元 水解 微生物 筛选 

分 类 号：R282.4[医药卫生—中药学]