桦褐孔菌三萜类物质的提取与含量测定  

Extraction and determination of triterpenoids from Phaeoporus obliquus

作　　者：林庚申[1] 张小青[1] 魏铁铮[1] 郭良栋[1] 

机构地区：[1]中国科学院微生物研究所真菌地衣系统学重点实验室

出　　处：《菌物学报》2007年第3期396-403,共8页Mycosystema

基　　金：中科院微生物研究所领域前沿青年基金(No.O654041011)

摘　　要：以桦褐孔菌发酵菌丝体为材料,通过对溶剂乙醇(95%)、甲醇、乙酸乙酯、丙酮、异丙醇、正己烷和氯仿的提取效果比较表明,提取三萜类化合物的最佳溶剂为异丙醇,提取时间为24h,每个样品所需溶剂量(6mL)和菌丝体样品量(100mg)较少,并可同时对大量样品进行有效提取。以白桦脂醇为标准品,对香草醛-冰醋酸-高氯酸分光光度法进行评价,证明该方法简单快速、准确度高、试验误差小、重复性好。利用此方法对桦褐孔菌的野生菌核和发酵菌丝体内三萜类化合物含量进行测定,结果表明野生菌核(59.86mg/g)和发酵菌丝体(94.92mg/g)中都含有很高的三萜类化合物,且发酵菌丝体中三萜类化合物含量高于野生菌核。因此在桦褐孔菌产品开发中,发酵菌丝体可代替野生菌核进行大工业化生产。A procedure of triterpenoid （TIP） extraction from Phaeoporus obliquus was developed. The result of screening of seven solvents proved that the optimal solvent for extraction of total TIP from P. obliquus was isopropanol with the extraction time of 24h. In this extraction method less isopropanol （6mL） and dry mycelia （100mg） were used and lots of samples were able to be extracted simultaneously. Using vanillin-acetic acid-perchloric acid spectrophotometry to detect total TTP in P. obliquus has proved to be simple, speedy, accurate, and repeatable. The detection result showed that TTP contents in natural sclerotia and fermentative mycelia were 59.86mg/g and 94.92mg/g respectively showing the TTP content in fermentative mycelia was higher than that in natural sclerotia. The results suggest that the product development of TTP from fermentative mycelia of P. obliquus can be an alternative to the collection of natural sclerotia of the fungus.

关 键 词：白桦脂醇 菌丝体 菌核 分光光度法 

分 类 号：R284[医药卫生—中药学]