马铃薯晚疫病菌DK98-1、DX98-2和DX98-3的生物学特性及AFLP指纹分析  

Biological characteristics and AFLP fingerprints of Phytophthora infestans strains DK98-1,DX98-2 and DX98-3

作　　者：朱杰华[1] 杨志辉[1] 刘大群[1] 刘顺[1] 

机构地区：[1]河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《菌物学报》2007年第3期455-462,共8页Mycosystema

基　　金：河北省自然科学基金(No.C2004000379);河北农业大学重点科技项目(No.9816)

摘　　要：利用黑麦培养基和V8-蔬菜汁培养基研究了马铃薯晚疫病菌Phytophthora infestans特异菌株DK98-1、DX98-2和DX98-3的生物学特性,发现该菌株与普通菌株相比菌落生长速度慢、孢子囊产生数量少、有性杂交后卵孢子产生量大(2047～75623个/cm2);利用AFLP分子标记研究这3个菌株的DNA指纹图谱,发现用引物E+CG/M+CC扩增菌株DK98-1、DX98-2和DX98-3后,在330bp处与普通菌株相比各缺失一条谱带,用引物E+AC/M+CT扩增菌株DK98-1、DX98-2和DX98-3后,在370bp处比普通菌株增加1条谱带,说明这3个菌株与普通菌株在遗传上明显不同。同时可以利用上述2对特异性引物,鉴定在自然界的晚疫病菌群体中这类特异菌株的出现频率。Biological characteristics of the Phytophthora infestans strains DK98-1, DX98-2 and DX98-3 have been studied on the rye medium and V8-vegetable juice medium. As compared with the common strains, the colony growth of these strains was slow, the quantity of sporangia was few, and the quantity of oospore（2047 75623/cm^2） produced was much more after sexual hybridization. DNA fingerprints of the 3 strains were detected by AFLP molecular marker. Strains DK98-1, DX98-2 and DX98-3 lack one band as compared with common strains on site of 330bp after they were amplified with the primer E＋CG/M＋CC, while they increase one band on site of 370bp after they were amplified with the primer E＋AC/M＋CT. It is indicated that these three strains distinguish from common strains at the genetic background. The frequency of the specific P. infestans strains could be investigated in nature by using these 2 specific primers.

关 键 词：致病疫霉 卵孢子 特异菌株 专一性引物 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]