伏马菌素B1对人外周血单个核细胞抗原加工相关转运子表达的影响  

Effects of Fumonisin on TAP-1 Expression of Human Peripheral Blood Mononuclear Cells in Vitro

作　　者：邢凌霄[1] 张祥宏[1] 李月红[1] 左连富[2] 严霞[1] 王俊灵[1] 王凤荣[1] 

机构地区：[1]河北医科大学病理研究室,石家庄市050017 [2]河北省肿瘤研究所细胞室,石家庄市050511

出　　处：《细胞生物学杂志》2007年第4期609-612,共4页Chinese Journal of Cell Biology

基　　金：河北省自然科学基金资助项目(No.C2004000613)~~

摘　　要：探讨伏马菌素B1(FB1)对体外培养的人外周血单个核细胞(hPBMC)抗原加工相关转运子(TAP-1)表达的影响。采用流式细胞定量检测(FCM)、免疫印迹(Western印迹)及半定量RT-PCR方法,研究不同浓度FB1(0,10和50μmol/L)处理后人外周血单个核细胞TAP-1在mRNA和蛋白质水平表达的影响。RT-PCR检测结果表明,10和50μmol/L FB1处理24h后,处理组细胞TAP-1mRNA明显低于对照组。在蛋白质水平,FCM定量分析表明,两个处理组细胞表面TAP1的平均荧光强度均较对照组降低,以50μmol/LFB1处理组降低显著(P<0.05)。免疫印迹结果亦表明,FB1处理组TAP-1的表达均较对照组降低。10和50μmol/LFB1可抑制体外培养的hPBMCTAP-1mRNA和蛋白质表达。To explore the effects of fumonisin （FB 1） on TAP- 1 （transporter associated with antigen processing） expression of human peripheral blood mononuclear cells （hPBMC） in vitro. The expression of TAP- 1 of hPBMC by FB1 pretreatment at three different dosages （0, 10 and 50 μmol/L） were detected with flow cytometry （FCM）, Western blot and semi-RT-PCR respectively. TAP- 1 mRNA was decreased in 10 and 50 μmol/L FB 1 treated groups than that in control group. FCM quantitative analysis showed that at protein level, TAP- 1 expression （as expressed by FI） of hPBMC at FB1 treated groups were lower than that of control, especially in 50 μmol/L FB1 treated groups （P〈0.05）. Western blot results further confirmed the above results. 10 and 50 μmol/L FB 1 treatment could inhibit the expression of TAP- 1 at mRNA and protein level.

关 键 词：伏马菌素B1 人外周血单个核细胞 抗原加工相关转运子 

分 类 号：R392[医药卫生—免疫学]