川芎嗪抗链霉素耳毒性作用及其对豚鼠耳蜗外毛细胞钾通道的影响(英文)  

Attenuation of streptomycin ototoxicity by tetramethylpyrazine and its effect on K^+ channels in the outer hair cells of guinea pig cochlea

作　　者：汤浩[1] 崔桂英[1] 石丽娟[1] 高青华[1] 曹宇[1] 

机构地区：[1]中国医科大学基础医学院生理学教研室,沈阳110001

出　　处：《生理学报》2007年第4期534-538,共5页Acta Physiologica Sinica

基　　金：This work was supported by the National Natural Science Foundation of China(No.39470246).

摘　　要：本文旨在研究川芎嗪(tetramethylpyrazine,TMP)拮抗链霉素耳毒性作用及其对豚鼠耳蜗外毛细胞K^+通道的影响,探讨TMP拮抗链霉素耳毒性的离子通道机制。60只豚鼠随机分为6组,应用听觉脑干反应(auditory brainstem response,ABR)技术检测豚鼠ABR听阈,观测TMP的抗链霉素耳毒作用;并采用全细胞膜片钳技术观察TMP对耳蜗外毛细胞Ca^(2+)敏感K^+电流的影响。结果显示,TMP明显降低链霉素导致的豚鼠ABR听阈升高,提示TMP具有抗链霉素耳毒性作用;TMP能明显增大豚鼠耳蜗外毛细胞Ca^(2+)敏感K^+电流,并呈浓度依赖关系。结果提示,TMP通过增大K^+通道电导而拈抗链霉素耳毒性作用。In order to elucidate the mechanism underlying the attenuation of streptomycin ototoxicity by tetramethylpyrazine （TMP）, the present study investigated the effect of TMP on the outward K^＋ current in the outer hair cells of guinea pig cochlea. Sixty guinea pigs were divided into 6 groups randomly. Auditory brainstem response （ABR） was used to observe the change in thresholds and to evaluate ototoxicity induced by streptomycin. Whole-cell patch-clamp technique was used to observe the effect of TMP on outward K^＋ current in isolated outer hair cells. The results showed that TMP attenuated the threshold shift caused by streptomycin and increased the amplitudes of Ca^2＋-sensitive K^＋ current [IK（Ca] in the outer hair cells. The present data suggest that TMP displays anti-ototoxicity induced by streptomycin. The augmented amplitudes of IK（Ca） of the outer hair cells induced by TMP may be one of the mechanisms underlying its ototoxicity-attenuating effect.

关 键 词：川芎嗪 外毛细胞 链霉素 K^+通道 

分 类 号：R285.5[医药卫生—中药学]