Imprinting Analysis of the Porcine MEST Gene in 75 and 90 Day Placentas and Prenatal Tissues  

作　　者：Chenchang XU Lijie SU Quanyong ZHOU Changchun LI Shuhong ZHAO 

机构地区：[1]Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education, and Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070,China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第8期633-639,共7页生物化学与生物物理学报（英文版）

基　　金：This research was supported lay the grants from the National Natural Science Foundation of China （30571328）, the Students Research Fund （SRF） of Huazhong Agricultural University （A06056）, the Key Project of the National Basic Research and Developmental Plan （2006CB102105） of China, the Program for New Century Excellent Talents in University （NCET-05-0669）, and the Hubei Province Natural Science Creative Team Project （2006ABC008）

摘　　要：Imprinted genes play important roles in mammalian growth, development and behavior. Mouse mesoderm-specific transcript （MEST） has been identified as an imprinted gene and mapped to an imprinted region of mouse chromosome 6 （MMU6）. It plays essential roles in embryonic and placental growth, and it is required for maternal behavior in adult female mouse. Here, we isolated the porcine MEST gene and detected a single nucleotide polymorphism in the 3＇-untranslated region. The RsaI polymorphism was used to investigate the allele frequencies in different pig breeds and the imprinting status in prenatal porcine tissues. Allele frequencies were significantly different between the native Chinese and Landrace breeds, except that most of the native Yushan pigs （21/26） are heterozygous at this locus. The results indicate that MEST was imprinted in placentas on days 75 and 90 of gestation as well as in the 75 d fetal heart, muscle, kidney, lung and liver.Imprinted genes play important roles in mammalian growth, development and behavior. Mouse mesoderm-specific transcript （MEST） has been identified as an imprinted gene and mapped to an imprinted region of mouse chromosome 6 （MMU6）. It plays essential roles in embryonic and placental growth, and it is required for maternal behavior in adult female mouse. Here, we isolated the porcine MEST gene and detected a single nucleotide polymorphism in the 3＇-untranslated region. The RsaI polymorphism was used to investigate the allele frequencies in different pig breeds and the imprinting status in prenatal porcine tissues. Allele frequencies were significantly different between the native Chinese and Landrace breeds, except that most of the native Yushan pigs （21/26） are heterozygous at this locus. The results indicate that MEST was imprinted in placentas on days 75 and 90 of gestation as well as in the 75 d fetal heart, muscle, kidney, lung and liver.

关 键 词：pig mesoderm-specific transcript gene IMPRINTING PLACENTA 

分 类 号：Q132.6[生物学—普通生物学]