针刺对新生鼠缺血缺氧性脑损伤皮质神经营养因子及受体基因表达的影响  

Effects of acupuncture on gene expression profile of neurotrophin and its receptors in cerebral cortex of neonatal rats after cerebral hypoxic-ischemic injury

作　　者：庄明华[1] 郭燕春[1] 白晔[2] 刘明发[1] 骆健明[1] 

机构地区：[1]汕头大学医学院第二附属医院神经外科,汕头515041 [2]汕头大学医学院第二附属医院针灸科,汕头515041

出　　处：《中华物理医学与康复杂志》2007年第8期530-533,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：广东省第三批科技计划项目(2003c33902)

摘　　要：目的观察针刺对新生大鼠缺血缺氧性脑损伤皮质神经营养因子及受体基因表达的影响,探讨针刺治疗缺血缺氧性脑病的分子机制。方法建立新生大鼠脑缺血缺氧性损伤模型,用GEArray Q系列神经营养因子及受体基因芯片检测针刺治疗14 d后大脑皮质基因表达的变化。结果针刺组共有48条基因(占被检测基因总数的50%)与对照组有表达差异,其中40条[占差异表达基因的83.3%,如脑源性神经营养因子(BDNF)、睫状神经营养因子(CNTF)及受体(CNTFR)、碱性成纤维细胞生长因子(bFGF)及受体(FG- FRI)等]上调,8条[占16.7%,如神经调节素1(Nrgl)、神经调节素4(Nrg4)及酪氨酸激酶C(TrkC)等]下调。结论针刺对缺血缺氧性脑损伤新生大鼠大脑皮质多种神经营养因子及受体基因的表达有调节作用,这可能介导了针刺治疗缺血缺氧性脑病的分子机制。Objective To investigate the effects of acupuncture on gene expression profile of neurotrophin and its receptors in cerebral cortex of neonatal rats after cerebral hypoxic-ischemic injury, and to explore the molecular mechanism of acupuncture in treatment of neonatal hypoxic-ischemic encephalopathy. Methods The model of cerebral hypoxic-ischemic injury was established with 10 neonatal Sprague-Dawley （SD） rats, who were subjected to acupuncture once daily for 14 days. The animals were sacrificed on the next day of the last acupuncture and their brain cortex was sampled for examination of gene expression, using GEArray Q series neurotrophin and receptors gene array. Results After 14 days of acupuncture, it was found that 48 genes （50% of total genes on the microarray） were expressed differently between the two groups, of which 40 genes （83.3% of differently expressed genes） , such as those of the brain-derived neurotrophic factor （BDNF） ,ciliary neurotrophic factor （CNTF） , ciliary neurotrophic factor receptor （CNTFR）, basic fibroblast growth factor （bFGF） and fibroblast growth factor receptor type 1 （FGFR1） were up-regulated, and 8 genes （16.7% of differently expressed genes）, such as genes of neuregulin-1 （ Nrgl ） , neuregulin-4 （Nrg4） and tyrosine kinase C （TrkC） were down-regulated. Conclusion Acupuncture can regulate the expression of various genes of neurotrophin and receptors in cerebral cortex of neonatal rats after cerebral hypoxic-ischemic injury, which might be the mechanism of acupuncture facilitating the recovery of the rats from hypoxic-ischemic encephalopathy.

关 键 词：针刺 缺血缺氧性脑病 神经营养因子 受体 

分 类 号：R245[医药卫生—针灸推拿学]