循环抗原及血清抗体的联合检测诊断日本血吸虫病  

The combined profile tests of circulating Sj14-3-3 and its antibodies for the diagnosis of schistosomiasis

作　　者：余轶婧[1] 沈继龙[1] 罗庆礼[1] 乔增培[1] 

机构地区：[1]安徽医科大学病原生物学教研室,教育部重要遗传病基因资源利用重点实验室(省部共建),省重要遗传病基因资源利用重点实验室,安徽医科大学人兽共患病研究所,合肥230032

出　　处：《安徽医科大学学报》2007年第4期351-354,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家高技术研究发展计划(863计划)项目(编号:2004AA2Z3570)

摘　　要：目的探讨日本血吸虫信号蛋白14-3-3(Sj14-3-3)在血吸虫病免疫诊断中的价值。方法制备并纯化抗重组日本血吸虫信号蛋白14-3-3(rSj14-3-3)的单抗和多抗,并建立双抗体夹心酶联免疫吸附试验(ELISA);以纯化的rSj14-3-3建立间接ELISA,分别比较该两种方法以及两种方法联合检测对于急、慢性血吸虫病患者免疫诊断的特异性和敏感性。结果利用双抗体夹心ELISA、间接ELISA和联合检测3种方法检测急性患者血清70例,敏感性分别为72.9%、75.7%和91.4%;检测慢性患者血清110例,敏感性分别为46.4%、61.8%和82.7%;检测正常人血清100例,特异性分别为96%、92%和92%;吡喹酮治疗后患者血清161份,其中急性治疗后1年以内转阴率分别为50%、38.9%和33.3%;慢性治疗后1年内分别是76.4%、55.6%和43.1%;慢性治疗后2年以上分别为93.0%、83.0%和78.9%。3种方法检测华支睾吸虫病、钩虫病的交叉反应性分别为0%、15.6%和15.6%,8.7%、19.6%和21.7%。3种方法的敏感性、与华支睾吸虫感染者血清交叉反应、慢性治疗1年内及慢性治疗2年以上患者血清的转阴率差异具有显著性(P<0.05),而特异性、与钩虫感染者血清交叉反应及其他化疗后患者血清的转阴率差异无显著性(P>0.05)。结论循环抗原Sj14-3-3和抗体联合检测在日本血吸虫病免疫诊断中具有潜在的临床应用价值。Objective To evaluate the immunodiagnostic value of the profile test system in detecting both circulating antigen Sj14-3-3 and its antibodies in the patients with schistosomiasis. Methods The rSj14-3-3 expression in E. coli was induced and purified through affinity chromatography. Monoclonal and polyclonal antibodies against rSj14-3-3 were produced. Sera of patients with acute and chronic schistosomiasis were detected with rSj14-3-3 indirect ELISA, double antibody sandwich ELISA（ using anti-rSjl4-3-3 monoclonal and polyclonal antibodies）, and the combined detections of Sj14-3-3 and its antibodies. Results The positive rates of the three methods in 70 cases of acute schistosomiasis were 72. 9% , 75.7% and 91.4% , while in 110 cases of chronic schistosomiasis were 46. 4% ,61.8% and 82. 7% ,respectively. The negative rates in 100 normal persons were 96% , 92% and 92% , while in 18 patients treated with praziquantel were 50% ,38.9% and 33.3%, respectively. The negative rates in 72 chronic cases with one year posttreatment were 76. 4%, 55.6% and 43.1%, respectively. The negative rates in 71 chronic subjects with over two years posttreatment were 93.0%, 83.0% and 78.9%, respectively. Cross reactions in sera from 64 cases of clonorchiasis were found to be 0% ,15.6% and 15.6% , respectively and from 46 cases of hockworm infection were 8.7%, 19. 6% and 21.7%, respectively. Statistically significant difference was noticed in the results, using rSj14-3-3 indirect ELISA, double antibody sandwich ELISA and the combined detection. Conclusion The profile tests of both circulating antigens and antibodies have a potential in immunodiagnoses of schistosomiasis.

关 键 词：血吸虫病 日本/诊断 14-3-3蛋白质类 抗原 蠕虫/分析 抗体 蠕虫/分析 

分 类 号：R341[医药卫生—基础医学] R383.24