rhIGF-1对神经细胞缺血再灌注损伤的保护作用  

Protective Effects of Recombinant Human IGF-1 on Neural Cell in Ischemia-Reperfusion Injury

作　　者：刘国萍[1] 郭洁[1] 黄维惠[1] 汪志云[1] 梁东春[2] 

机构地区：[1]天津市第一中心医院神经内科,300192 [2]天津医科大学内分泌研究所

出　　处：《天津医药》2007年第9期675-677,723,共4页Tianjin Medical Journal

基　　金：天津市自然科学基金重点项目(项目编号:033801611)

摘　　要：目的:研究重组人胰岛素样生长因子1(rhIGF-1)对缺血再灌注所致神经细胞损伤的保护作用。方法:自新生大鼠脑组织中分离培养原代神经细胞,3H-TdR掺入法检测rhIGF-1对神经细胞增殖的影响。将所培养的细胞分为正常对照组、类缺血对照组及损伤后rhIGF-1干预组,对不同组别的细胞进行类缺血-复氧处理,流式细胞检测细胞凋亡情况。结果:rhIGF-1可明显促进原代培养的神经细胞增殖,并降低再灌注损伤所导致的细胞凋亡。结论:rhIGF-1对神经细胞缺血再灌注损伤具有保护及治疗作用。Objective： To study the protective effects of recombinant human IGF-1 （rhIGF-1） on neural cell in ischemia-reperfusion injury. Methods： Neural ceils were separated from the brain of neonatal SD rats. The effects of rhIGF-1 on ceil proliferation were detected by 3H-TdR incorporation method. Ceils were randomly divided into 3 groups including normal group, hypoxia control group and rhIGF-1 treatment group. Ischemia-reperfusion was performed in hypoxia control group and rhIGF-1 treatment group. Apeptosis rate was detected by flow cytometry. Results： rhIGF-1 promoted the proliferation of primary cultured neurons and decreased the apeptosis rate caused by ischemia-reperfusion. Conclusion： rhIGF-1 has protective and therapeutic effects on neurons in ischemia-reperfusion injury.

关 键 词：胰岛素样生长因子Ⅰ 神经元 细胞 培养的 再灌注损伤 细胞凋亡 

分 类 号：R657.3[医药卫生—外科学]