镉对诱导成熟人树突状细胞的毒性作用  

The cytotoxic effect of cadmium on human matured dendritic cells

作　　者：梁嘉斌[1] 林育纯[1] 方飞[1] 陈雯[1] 陈成章[1] 林忠宁[1] 

机构地区：[1]中山大学公共卫生学院预防医学系,广东广州510080

出　　处：《中国职业医学》2007年第4期265-267,270,共4页China Occupational Medicine

基　　金：国家自然科学基金资助课题(No.30471428);广东省自然科学基金资助课题(No.05001765)

摘　　要：目的 探讨镉化合物对人树突状细胞（DC）的细胞毒性作用。方法 应用粒-巨噬细胞集落刺激因子（GM-CSF）＋白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α）诱导人外周血单核细胞获得DC，流式细胞仪（FACS）检测细胞表面标记鉴定；给予CdCl2处理进行剂量-效应和时间-效应分析，采用甲基四氮唑盐（MTS）颜色反应法检测细胞毒性作用。结果 诱导获得的DC呈悬浮生长，部分细胞聚集成簇。细胞表面标志分子表达阳性CD14为2.7%，HLA-DR为96.3%，CD1a为83.9%，CD80为88.3%，CD83为58.8%，CD86为42.4%。不同剂量（1.25-80.00μmol/L）和作用时间（24-48h）CdCl2染毒组细胞增殖抑制率不同程度增高，呈剂量-和时间-依赖性关系。结论 诱导获得成熟的人DC；镉作用可致成熟DC的细胞毒性效应。Objective To study the cytotoxicity of cadmium compound on dendritic cells （DC）. Methods Human peripheral blood monocytes （ PBMC ） from healthy donors were induced with GM-CSF ＋ IL-4 and TNF-α to harvest DC. The surface markers on DC were analyzed by Flow cytometry. The MTS assay was conducted to evaluate the proliferative function of matured DC treated by CdCl2 in a dose-effect （ 1.25 - 80. 00 μmol/L） and time-course （ 24 - 48 hours ） studies. Results The induced DC growed suspendedly and aggregated into clumps with typical morphology. There was high level expression simultaneously in HLADR （96. 3% ）, CD1a （83.9%）, CDSO （88.3%）,CD83 （58.8%） and CD86 （42. 4%）, hut low level in CD14 （2. 7%）. The cellular proliferation inhibition rate of DC significantly increased in cadmium-treated groups in dose- and time-dependent patterns. Conclusion It was shown that there was inducible cytotoxic effect of CdCl2 on human matured DC.

关 键 词：镉 树突状细胞 免疫细胞毒性 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]