合成聚肽嵌段共聚物PHEG-PBLG结构的核磁共振分析  

Synthetic Polypeptide Structure of Block Copolymer PHEG-PBLG Analyzed by NMR

作　　者：张静夏[1] 李碧虹[2] 王琴梅[2] 黄爱东[1] 

机构地区：[1]中山大学药学院,广东广州510089 [2]中山大学附属第一医院,广东广州510089

出　　处：《波谱学杂志》2007年第3期267-273,共7页Chinese Journal of Magnetic Resonance

基　　金：广东省自然科学基金资助项目(031684).

摘　　要：通过L-谷氨酸苄酯和三光气反应制备谷氨酸苄酯的N-羧酸酐,以聚羟乙谷氨酸酰胺为大分子引发剂,投入不同配比的单体/引发剂(A/I),共聚得到两亲性嵌段共聚物聚羟乙谷氨酰胺-聚谷氨酸苄酯(PHEG-PBLG);通过核磁共振技术分析表征该合成聚肽的结构组成、分子量范围及螺旋结构;通过体外酶解实验研究了共聚物的降解情况.研究结果表明:该合成聚肽化合物为两亲性嵌段聚合物:当聚合物中亲水链段PHEG分子量为9000,A/I为75∶1,100∶1,150∶1,200∶1时,相应的共聚物的分子量分别为:1.27×104,1.75×104,1.9×104,3.60×104;该合成聚肽含有α-螺旋结构,随着TFA的加入,该聚肽的结构由α-旋构象转化为随机盘绕构象.Initiated by poly（hydroxylethyl glutamine）, amphiphilic block copolymer of poly（hydroxylethyl glutamine （PHEG）） and poly（γ-benzyl-L-glutamate （PBLG）） was synthesized by polymerization of N-carboxyl anhydride （NCA）. NCA monomer was synthesized by reacting γ-benzyl-L-glutamate with triphosgene. The constitution, molecular weight and a-helical structures of the resulting block copolymers were studied by NMR and enzymolysis of the black polymers in vitro was discussed. The results showed that the copolymers with different block-ratio of PHEG/PBLG had different molecular weights, being 1.27×10^4,1.75×10^4 ,1.9×10^4and 3.60×10^4 when the A/I ratios were 75 ： 1, 100 ： 1,150 ： 1 and 200： 1, respectively. The copolymers contained α-helix structures in CHCl3, which could be converted into random coils with addition of TFA.

关 键 词：核磁共振 生物材料 合成聚肽 聚羟乙谷氨酰胺-聚谷氨酸(PHEG-PBLG) 

分 类 号：O631.1[理学—高分子化学]