和络舒肝胶囊对肝纤维化肝组织中乙酰胆碱受体的影响  

EFFECT OF HELUOSHUGAN CAPSULES ON ACETYLCHOLINE RECEPTOR IN LIVER TISSUE FIBROSIS

作　　者：郭燕[1] 高啸[1] 晏维[1] 张芳杰[1] 唐望先[1] 

机构地区：[1]华中科技大学同济医学院同济医院肝病研究所,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2007年第2期154-158,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(30440088)

摘　　要：目的探讨和络舒肝胶囊对CCl4诱导的肝纤维化模型的肝脏中乙酰胆碱受体的影响及其抗肝纤维化的可能机制。方法应用四氯化碳(CCl4)诱导大鼠慢性肝纤维化模型。38只Wistar大鼠随机分为正常对照组(N组),肝纤维化模型组(M组)和和络舒肝组(H组)。应用逆转录聚合酶链反应(RT-PCR)和免疫印迹(Western Blot)方法检测肝脏组织中乙酰胆碱受体在mRNA及蛋白质水平的表达情况。结果正常组肝组织内乙酰胆碱受体含量明显低于模型组(P<0.01),且和络舒肝组乙酰胆碱受体含量低于模型组(P<0.05)。结论和络舒肝胶囊的作用可能与降低肝纤维化肝组织中乙酰胆碱受体的含量有关。Objective To investigate the effect of heluoshugan capsules on acetylcholine receptors and their possible mechanism in resisting hepatic fibrosis induced by carbon tetrachloride （CCL）. Methods 38 rats were randomly divided into the control group, model group and HLSG-treated group. The acetylcholine receptor concentrations in liver tissue were determined with RT-PCR and Western blot. Results The level of acetylcholine receptors was obviously lower in the control group than in the model group （P 〈0.01）, and lower inthe HLSG-treated group than in the model group （P〈0.05）. Conclusion Heluoshugan capsule could decrease the acetylcholine receptor concentration in liver tissue of fibrosis.

关 键 词：和络舒肝胶囊 肝纤维化 乙酰胆碱受体 神经递质 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]