α-SMA在培养生长板软骨细胞中的表达  

EXPRESSION OF ALPHA-SMA IN CULTURED GROWTH PLATE CHONDROCYTE

作　　者：季煜华[1] 曾耀英[1] 胡萍[2] 肇静娴[1] 

机构地区：[1]暨南大学组织移植与免疫研究中心 [2]暨南大学医学院免疫学教研室,广州510632

出　　处：《中国组织化学与细胞化学杂志》2007年第2期195-199,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：973国家重点基础研究发展规划项目(G1999054303);国家自然科学基金(3060058);广东省"十五"重大科技专项(A302020204);暨南大学引进人才启动基金(51205066);广州市科技计划项目(2002J1-C0361)

摘　　要：目的探讨α-SMA在原代培养生长板软骨细胞中的表达及传代培养对其表达的影响。方法分离、培养大鼠肋生长板软骨细胞(rat costochondral growth plate chondrocyte,RGC),免疫细胞化学(ICC)和Western blot分别对1-5代RGC中α-SMA的表达进行定性和半定量分析。结果原代培养的RGC不表达α-SMA,随着传代次数的增加,α-SMA阳性染色的细胞比例从原代的0增加到第5代的24.2±4.3%(n=3),Western blot结果与ICC结果一致。结论本研究首次发现原代培养的RGC并不表达α-SMA,随着RGC传代次数的增加,表达α-SMA的细胞比例和表达量不断升高。Objective To investigate α-SMA （alpha-smooth muscle actin） expression in primary cultured growth plate chondrocytes, as well as the influence of serial passage on α-SMA expression. Methods Isolated and cultured rat costochondral growth plate chondrocytes （RGCs） α-SMA and collagen type Ⅱ expression were qualitatively and semi-quantitatively analyzed with ICC and Western blot. Results Primary cultured RGCs were α-SMA negative. However, as the passage number increased, the number of α-SMA positive RGCs increased to 24. 2±4. 3% （n=3） of passage 5. Western blot results were consistent with ICC results. Conclusion This is the first report that primary cultured RGCs do not express α-SMA. How- ever, with increasing passages both the ratio of α-SMA positively RGCs and the quantities of expressed α- SMA rise.

关 键 词：生长板软骨细胞 Α-平滑肌肌动蛋白 免疫细胞化学 WESTERN BLOT 

分 类 号：R322.7[医药卫生—人体解剖和组织胚胎学]