硝酸羟胺对大鼠脑中Synaptobrevin和Syntaxin的表达的影响  

THE EXPRESSION CHANGES OF SYNAPTOBREVIN AND SYNTAXIN IN RAT BRAIN TISSUES CAUSED BY INJECTION OF HYDROXYLAMMONIUM NITRATE

作　　者：高亚兵[1] 彭瑞云[1] 王水明[1] 王德文[1] 陈建魁[2] 马俊杰[1] 张志毅[3] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]解放军307医院 [3]军事医学科学院仪器测试中心,北京100850

出　　处：《中国组织化学与细胞化学杂志》2007年第3期274-277,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的观察硝酸羟胺(HAN)对大鼠脑组织Synaptobrevin 2、Syntaxin的影响,探讨二者在HAN致脑损伤中的作用。方法应用免疫荧光双标记技术标记Synaptobrevin2和Syntaxin,用Hochest衬染细胞核,Radiance2100型激光共聚焦显微镜观察。结果Synaptobrevin 2及Syntaxin广泛分布在正常大脑皮质、海马及小脑,HAN注射后7d内,Synaptobrevin 2表达减弱,Syntaxin表达增强。结论Synaptobrevin 2和Syntaxin比例失调和相互作用减弱可能在HAN致脑损伤中发挥重要作用。Objective To study the expressions of Synaptobrevin 2 and Syntaxin in rat brain tissues and the damage mechanisms after the injection of Hydroxylammonium nitrate （HAN）. Methods The antibodies to Synaptobrevin 2 and Syntaxin were labeled with red and green fluorescence dyes respectively.The cell nuclei were stanined with Hochest. The results were observed with Radiance2100 laser scanning confocal microscopy. Results Synaptobrevin 2 and Syntaxin were widely distributed in the normal cerebral cortex, hippocampus and cerebellum. The expression of Synaptobrevin 2 was up-regulated and that of - Syntaxin was down-regulated in the brain 7d after HAN injection. Conclusion The expression disorder of Syntaxin was down-regulated in the brain 7d after HAN injection. Conclusion The expression disorder of Synaptobrevin 2 and Syntaxin may play an important role in the damages of brain tissue by the administration of hydroxylammonium nitrate.

关 键 词：免疫荧光技术 硝酸羟胺 激光共聚焦扫描显微镜 Synaptobrevin2 SYNTAXIN 

分 类 号：R595[医药卫生—内科学]