3个仿刺参地理种群遗传变异的微卫星DNA分析  

Genetic diversity of three geographical populations of Apostichopus japonicus revealed by microsatellite DNA

作　　者：谭杰[1] 孙慧玲[1] 刘萍[1] 杨爱国[1] 燕敬平[1] 刘志鸿[1] 周丽青[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所

出　　处：《水产学报》2007年第4期437-442,共6页Journal of Fisheries of China

基　　金：山东省科技攻关项目(022110114;2005GG3205070);农业结构调整重大技术研究专项(06-05-04B)

摘　　要：运用微卫星DNA技术对仿刺参烟台群体、威海群体、大连群体的3个野生群各20个个体进行了遗传分析。对9个基因位点进行了扩增，共获得了59个等位基因。评估了9对微卫星引物的多态信息含量（PIC），范围在0．5129～0．8794之间。结果表明：3个野生群体的平均杂合度观测值分别为0．6416、0．6595、0．5824，平均杂合度期望值分别为0．7641、0．7161、0．7364；在Hardy-Weinberg平衡条件下，进行了P检验，发现3个群体均有位点发生了显著偏离；对3个群体进行了配对Fst值计算，发现3个群体之间遗传分化较弱。从变异贡献率来看，95．68％的变异来自个体之间，4．32％的变异来自群体之间。经过聚类分析，发现威海群体和大连群体之间亲缘关系较近，烟台群体与前两群体亲缘关系较远。The microsatellite DNA technique was applied to assess genetic diversity and genetic differentiation in three wild populations of Apostichopus japonicus from Yantai, Weihai and Dalian. 9 polymorphic loci were screened electrophoretically for genetic variation of the three wild populations. The following parameters were calculated： heterozygosity, polymorphism information content （PIC）, effective allele number, allele frequency, genetic distance, genetic identity coefficient, Hardy-Weinberg balance deflection index and so on. Results showed that the number of total alleles of these 9 microsatellite loci was 59. The average of allele number in each microsatellite locus of the Yantai, Weihai and Dalian populations was 6.5556,5.8889 and 6.0000, respectively; the average effective allele number was 4.6033,4.0466 and 4. 1367, respectively; The PIC of polymorphic loci varied between 0. 5129 - 0. 8794. The average observed heterozygosity （ Ho ） of three populations was 0.6416, 0.6595 and 0.5824 respectively, and the average expected heterzygosity（ He ） was 0. 7641,0. 7161 and 0. 7364, respectively. Cluster analysis of the three wild populations performed with UPGMA （unweighted pair groupmethod with arithmetic mean confirmed the closer linkage between Weihai and Dalian than their individual distances to Yantai.

关 键 词：仿刺参 地理群体 遗传变异 微卫星DNA 

分 类 号：Q347[生物学—遗传学] S917.4[农业科学—水产科学]