Induction of corneal epithelial progenitors from bone-marrow mesenchymal stem cells of rhesus monkeys in vitro  

作　　者：YUAN Jing YU JianXiong HUANG Bing LIU BingQian LIU JingBo JIANG RuZhang GE Jian 

机构地区：[1]State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China [2]Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University, Guangzhou 510080, China [3]Department of General Surgery, The Third Affiliated Hospital of SUR Yat-sen University, Guangzhou 510630, China

出　　处：《Chinese Science Bulletin》2007年第16期2216-2225,共10页

基　　金：Supported by the Key Clinical Program of the Ministry of Health of China (Grant No. 2004468);the National Natural Science Foundation of China (Grant No. 30672275);Natural Science Foundation of Guangdong Province (Grant No. 06300679);the Post-doctor Foundation of China (Grant No. 2005037616)

摘　　要：Bioengineered corneas are substitutes for human donor tissue that are designed to treat severe dis-ease affecting ocular surfaces. However,a shortage of candidate seed cells for bioengineering corneas is still a problem. Bone-marrow mesenchymal stem cells (MSCs) are capable of multilineage differen-tiation. Therefore,we determined whether MSCs differentiate into corneal epithelial cells (ECs). We applied three exoteric-microenvironmental systems to induce MSCs to become ECs. Induced MSC were identified by means of morphologic examination,immunocytochemical analysis,and flow cytometry. MSCs grown in one microenvironment had characteristics similar to those of corneal epithelial pro-genitors. Induced MSCs expressed markers for EC,including integrin β1,Cx43,Pax6,and P63. MSCs were successfully induced to become corneal epithelial progenitors. Therefore,the use of MSCs may hold substantial promise for reconstructing the ocular surface after corneal injury.Bioengineered corneas are substitutes for human donor tissue that are designed to treat severe disease affecting ocular surfaces. However, a shortage of candidate seed cells for bioengineering corneas is still a problem. Bone-marrow mesenchymal stem cells （MSCs） are capable of multilineage differentiation. Therefore, we determined whether MSCs differentiate into corneal epithelial cells （ECs）. We applied three exoteric-microenvironmental systems to induce MSCs to become ECs. Induced MSC were identified by means of morphologic examination, immunocytochemical analysis, and flow cytometry. MSCs grown in one microenvironment had characteristics similar to those of corneal epithelial progenitors. Induced MSCs expressed markers for EC, including integrin 61, Cx43, Pax6, and P63. MSCs were successfully induced to become corneal epithelial progenitors. Therefore, the use of MSCs may hold substantial promise for reconstructing the ocular surface after corneal injury.

关 键 词：猕猴 角膜内皮细胞 骨髓间叶干细胞 生长因子 脂多糖 

分 类 号：Q25[生物学—细胞生物学]