缺氧对大鼠皮层、海马NMDA受体NR1亚单位磷酸化的影响  

Effect of hypoxia on NR1 subunit of NMDA receptor in rat cortex and hippocampus

作　　者：吴喜贵[1] 赵延东[1] 阮怀珍[1] 

机构地区：[1]第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所,重庆400038

出　　处：《第三军医大学学报》2007年第18期1742-1745,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30470557);全军医学科研"十一五"计划面上项目(06MA196)~~

摘　　要：目的观察缺氧模型大鼠皮层、海马NMDA受体NR1亚单位酪氨酸磷酸化的变化。方法成年大鼠置于模拟海拔5500m高度的低压氧舱内,每天缺氧8h,分别缺氧3、7、14d和21d。采用免疫组化、Western blot+免疫共沉淀方法检测皮层、海马NR1及其酪氨酸磷酸化的变化。结果免疫组化显示缺氧皮层、海马NR1表达都明显高于对照组(P<0.01)。Western blot检测进一步证明NR1的表达显著高于对照组(P<0.01),且随着缺氧时间的延长而增加。缺氧各组酪氨酸磷酸化水平亦显著高于对照组(P<0.01),缺氧14d后酪氨酸磷酸化达到最高峰,而后开始下降,21d时仍显著高于对照组(P<0.01)。结论高原缺氧条件下大鼠皮层、海马NMDA受体NR1亚单位表达及酪氨酸磷酸化水平显著增加,酪氨酸磷酸化的变化可能是影响NMDA受体功能的主要原因。Objective To observe the expression and phosphorylation of N-methyl-D-aspartate （NMDA） receptor NR1 subunit in rat cortex and hippocampus under high altitude hypoxia. Methods The adult SD rats were exposed to hypobaric hypoxia imitating 5 500-meter high altitude for 8 h daily for 3, 7, 14, 21 d. Immunohistochemical staining, Western blotting and co-immunoprecipitation were used to detect the expression and phosphorylation of NR1 in rat cortex and hippocampus. Results Both immunohistochemistry and Western blotting showed NR1 expression in rat cortex and hippocampus was increased under hypoxia in a time-dependent manner. The tyrosine phosphorylation of hypoxia groups was increased, and reached the peak on day 14 after hypoxia, then decreased, still higher than that of control groups till day 21 （P 〈 0. 01 ）. Conclusion The increase of tyrosine phosphorylation of NR1 subunit in rat cortex and hippocampus under hypoxia may be a key influencing factor for NMDA receptor function.

关 键 词：缺氧 皮层 海马 NMDA受体NR1亚单位 磷酸化 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]