氟他胺致胚胎期雄性大鼠睾丸毒性的基因表达谱的研究  

Gene expression profiles of toxicated fetal rat testis exposed to flutamide

作　　者：李岩[1] 张浩[2] 吴德生[2] 

机构地区：[1]遵义医学院预防医学教研室,遵义563003 [2]四川大学华西公共卫生学院环境卫生学教研室,成都610041

出　　处：《第三军医大学学报》2007年第18期1752-1754,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金重点项目(30030120);纽约中华医学会资助(2005)~~

摘　　要：目的利用基因芯片技术研究雄激素干扰物氟他胺睾丸毒性效应的分子机制。方法对F0亲代母鼠于孕12～17d皮下注射染毒,于胚胎期20d解剖孕鼠,取出实验组与对照组雄性胎鼠睾丸,提取mRNA,分别用Cy5和Cy3探针逆转录标记,与Rat12KcDNA表达谱芯片杂交,荧光扫描分析;另将出生第2天的雄性子代断颈取血清,用睾酮试剂盒测定激素水平。结果芯片结果显示实验组差异表达基因共31条,其中下调基因14条,已知功能基因9条;上调基因17条,已知功能基因7条;一共16条差异表达基因与睾丸组织功能及发育密切相关;且检测睾酮激素水平下降。结论基因芯片技术能够为我们在分子水平上提供氟他胺对胚胎期雄性大鼠睾丸毒性损伤机制的线索。Objective To study the toxic effect of flutamide （Flu） on testis with DNA chip. Methods Flu was injected subcutaneously at doses of 6.25 mg · kg^-1·d^-1 to 25 F0 rats from gestation day （GD） 12 to 17, and the 25 rats of control group received same doses of normal saline. All 50 pregnant SD rats were sacririced on GD 20. The cDNA were extracted respectively from fetal testis, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently, cDNA probes were hybridized to the Mouse40S cDNA microarray and fluorescence signals were analyzed. Testosterone level was determined by enzyme-linked immunosorbent assay （ELISA）. Results Compared with control, a total of 31 identified gene was found to be expressed differently, including 14 down-regulated and 17 up-regulated. Among them, 5 down-regulated and 10 up-regulated genes were unknown in Genbank. ELISA indicated that testosterone levels were decreased in Flu toxic group. Conclusion Flu treatment to F0 pregnant rats can damage apparently to the reproductive development of embryonic period male SD rats. The obtained differential genes may provide reference for the molecular mechanism of reproductive development.

关 键 词：氟他胺 睾丸 基因芯片 

分 类 号：R12[医药卫生—环境卫生学] R322.64[医药卫生—公共卫生与预防医学]