Spectrofluorimetric Study on the Weak Interaction between ATP and Na-4-Tosyl-L-arginine Methyl Ester Hydrochloride  

作　　者：马彦青 吕功煊 李颖 刘书花 鲜亮 

机构地区：[1]Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China [3]Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China

出　　处：《Chinese Journal of Chemistry》2007年第9期1253-1258,共6页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No. 90210027）.Acknowledgments The authors especially thank Zhide Hu in Lanzhou University for kindly allowing the use of the RF-5301PC spectrofluorophotometer （Shimadzu） in this work and Wenying He in Lanzhou University for active discussion as well as zealous help during the fluorescence experiments.

摘　　要：In this paper, some new results on the selective weak interaction between Na-4-tosyl-L-arginine methyl ester hydrochloride （TAME） and adenosine-5＇-triphosphate （ATP） have been reported. Fluorescence spectrophotometry and Fourier transform infrared （FT-IR） spectroscopy were used to investigate this kind of weak interaction. In fluorescence experiments, obvious fluorescence quenching phenomena were observed when TAME was added, which indicated the weak interactions between TAME and ATP. It has been identified by fluorescence titration experiments that TAME exhibited high selectivity to ATP over ADP and AMP. FT-IR spectral results showed that an ATP-TAME adduct was formed. The experimental results indicated that the interaction sites were the guanidinium group of TAME main-chain and the γ-phosphate group of ATP, and the interaction took place through hydrogen bonding and electrostatic force. In addition, the effects of metal ions on the weak interaction between ATP and TAME, or between ATP and analogues of L-arginine were studied.In this paper, some new results on the selective weak interaction between Na-4-tosyl-L-arginine methyl ester hydrochloride （TAME） and adenosine-5＇-triphosphate （ATP） have been reported. Fluorescence spectrophotometry and Fourier transform infrared （FT-IR） spectroscopy were used to investigate this kind of weak interaction. In fluorescence experiments, obvious fluorescence quenching phenomena were observed when TAME was added, which indicated the weak interactions between TAME and ATP. It has been identified by fluorescence titration experiments that TAME exhibited high selectivity to ATP over ADP and AMP. FT-IR spectral results showed that an ATP-TAME adduct was formed. The experimental results indicated that the interaction sites were the guanidinium group of TAME main-chain and the γ-phosphate group of ATP, and the interaction took place through hydrogen bonding and electrostatic force. In addition, the effects of metal ions on the weak interaction between ATP and TAME, or between ATP and analogues of L-arginine were studied.

关 键 词：Na-4-tosyl-L-arginine methyl ester hydrochloride adenosine-5＇-triphosphate （ATP） binding site fluorescence quenching Fourier transform infrared （FT-IR） spectra 

分 类 号：O62[理学—有机化学]