Degradation of MTBE and TBA by a new isolate from MTBE-contaminated soil  

作　　者：ZHANG Rui-ling HUANG Guo-qiang LIAN Jing-yan LI Xin-gang 

机构地区：[1]School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China [2]National Engineering Research Center for Distillation Technology, Tianjin 300072, China [3]School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300191, China

出　　处：《Journal of Environmental Sciences》2007年第9期1120-1124,共5页环境科学学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(No.20276048);the Municipal Natural Science Foundation of Tianjin(No.06YFJMJC06800).

摘　　要：Methyl ten-butyl ether （MTBE）, a gasoline additive, possesses serious problems to the environmental health. In the present study, a bacterial culture named A-3 which could effectively degrade MTBE was isolated from the MTBE contaminated soil. The isolate was identified as Chryseobacterium sp., a new species capable of degrading MTBE. In order to enhance its degradation ability, selected environment factors were investigated. The results showed that the optimal temperature was in the range of 25-30℃, the pH was 7.0, the inoculum size was 2 × 10^8 CFU/ml and the optimal concentration of MTBE was from 50 to 100 mg/L. The maximum MTBE utilization rate （Vmax） was 102 nmol MTBE/（mg cell protein.h）. Furthermore, it was found that the isolate could also degrade tert-butyl alcohol （TBA）. The degradation rates of TBA were much faster than those of MTBE. The additional TBA would lead to the decrease of the initial MTBE degradation rate and the inhibitory effect of TBA increased with the increase of TBA concentration. Similar protein profiles at least seven peptides were demonstrated after SDS-PAGE analysis of crude extracts obtained from the cells growing in MTBE and TBA culture.Methyl ten-butyl ether （MTBE）, a gasoline additive, possesses serious problems to the environmental health. In the present study, a bacterial culture named A-3 which could effectively degrade MTBE was isolated from the MTBE contaminated soil. The isolate was identified as Chryseobacterium sp., a new species capable of degrading MTBE. In order to enhance its degradation ability, selected environment factors were investigated. The results showed that the optimal temperature was in the range of 25-30℃, the pH was 7.0, the inoculum size was 2 × 10^8 CFU/ml and the optimal concentration of MTBE was from 50 to 100 mg/L. The maximum MTBE utilization rate （Vmax） was 102 nmol MTBE/（mg cell protein.h）. Furthermore, it was found that the isolate could also degrade tert-butyl alcohol （TBA）. The degradation rates of TBA were much faster than those of MTBE. The additional TBA would lead to the decrease of the initial MTBE degradation rate and the inhibitory effect of TBA increased with the increase of TBA concentration. Similar protein profiles at least seven peptides were demonstrated after SDS-PAGE analysis of crude extracts obtained from the cells growing in MTBE and TBA culture.

关 键 词：methyl ten-butyl ether （MTBE） BIODEGRADATION ten-butyl alcohol （TBA） 

分 类 号：X53[环境科学与工程—环境工程] X13