低剂量 γ 射线照射后骨髓细胞抗自由基损伤的兴奋效应  

Hormesis of Free Radical Detoxiication in Bone Marrow Cells after Low dose γ ray Radiation

作　　者：邓文[1,2,3] 李毓琦[1,2,3] 余英[1,2,3] 林学东 雷厚成 唐爱华[1,2,3] 傅文茜 

机构地区：[1]四川省劳动卫生职业病防治研究所 [2]华西医科大学药学院 [3]华西医科大学第二附属医院

出　　处：《中国公共卫生学报》1997年第1期56-58,共3页

基　　金：四川省卫生厅科研基金

摘　　要：２和１０ｍＧｙ６０Ｃｏγ射线对５～６周龄Ｗｉｓｔａｒ品系雄性大鼠进行全身照射后４ｈ，动物骨髓细胞中还原型谷胱甘肽（ＧＳＨ）浓度、超氧化物歧化酶（ＳＯＤ）活性显著高于平等对照组。１０ｍＧｙγ射线照射后４ｈ，骨髓细胞中谷胱甘肽过氧化物酶（ＧＳＨＰｘ）活性显著升高。１００ｍＧｙγ射线照射后４ｈ未发现上述效应。２～１００ｍＧｙγ射线照射后动物骨髓细胞中超氧阴离子自由基（Ｏ－２）浓度、过氧化氢酶（ＣＡＴ）活性未见显著变化。在首次１０ｍＧｙγ射线对动物全身照射后４ｈ，再次用相同剂量进行重复照射，骨髓细胞中ＧＳＨ浓度、ＧＳＨＰｘ、ＳＯＤ活性立即恢复到对照水平。由于ＧＳＨ、ＧＳＨＰｘ、ＳＯＤ是细胞中抗自由基损伤的重要物质，本研究结果显示动物首次受低剂量γ射线照射后，骨髓细胞抗自由基损伤系统的防御反应增强即兴奋效应，对相继的第二次照射产生了防卫作用。抗自由基损伤系统的兴奋效应可能与细胞遗传学适应性反应存在着相关性。Four hours after irradiation of 5￣6 week male wistar rats with 2 and 10 mGy 60 Co γ rays,the concentrations of GSH and the activities of SOD in murine bone marrow cells increased significantly.GSHP x activities also increased significantly 4h after 10 mGy γ ray irradiation.The concentrations of O -· 2 and the activities of CAT remained unchanged 4h after irradiation with 2￣100mGy γ rays.Rats were again exposured to 10mGy γ rays 4h after the first 10mGy exposure.The results showed that the first exposure conditioned the cells in such a way that no effect at all over control was observed after the second exposure.With the view that GSH,GSHP x,SOD are essential to free radical detoxification,it can be infered from this study that low dose γ ray radiation could induce hormesis in free radical detoxification system in bone marrow cells.The comparison of results of this study and other laboratories suggested that the hormonesis in free radical detoxification system was possibly related to adaptive responses in cellular genetics.

关 键 词：低剂量辐射 骨髓细胞 自由基损伤 兴奋效应 

分 类 号：R818.02[医药卫生—放射医学]