甘蔗突变体AFLP分析  

AFLP analysis of sugarcane mutants

作　　者：黄玉辉[1] 陈涛[2] 吴晓伟[2] 杨荣仲[2] 李杨瑞[3] 

机构地区：[1]广西农业科学院蔬菜研究中心,广西南宁530007 [2]广西甘蔗研究所 [3]广西农业科学院,广西南宁530007

出　　处：《西南农业学报》2007年第4期727-731,共5页Southwest China Journal of Agricultural Sciences

基　　金：广西自然科学基金项目(桂科自0342007-1);广西农科院科技发展基金项目(2002020)

摘　　要：用桂糖11号作供体,经过诱变获得了在节间形状、叶型、芽形、生长带颜色等性状发生变异的突变体。用AFLP分子标记对这些突变体进行分析表明,突变体与供体之间存在着不同程度的多态性,多态位点数量随突变体不同而异,且多数突变体都存在多个位点突变。在突变体和供体之间,找到两个稳定的特异性AFLP标记,分别为桂糖11变引物EACC+MCAC,EACC+MCTG和EACT+MCCC的1 kb AFLP标记;桂糖11变和共培芽引物EACC+MCTG和EACT+MCCC的630bp AFLP标记。With GT 11 as donor, the mutants were produced with variations at intemode shape,leaf shape, bud shape, the colour of growth ring. The results of AFLP analysis for the mutants showed that there were polymorphism between mutants and donor,and the numbers of locus for the polymorphism were different among the mutants and many mutants had more than one mutation loci. Two AFLP markers were found in the 2 detected mutants. A 1Kb AFLP marker which was amplified by the primers EACC ＋ MCAC, EACC ＋ MCTG and EACT ＋ MCCC was found in the mutant of the GTll Bian. A 630bp AFLP marker which was amplified by the primers EACC ＋ MCTG and EACT ＋ MCCC was specifically related with the mutant of the GT11 Bian and the Gongpeiya.

关 键 词：甘蔗 突变体 AFkP 

分 类 号：S566.1[农业科学—作物学]