猪伪狂犬病病毒gD基因的克隆与分析  

Cloning and analysis of PRV-gD gene

作　　者：朱玲[1] 许雁峰[1] 郭万柱[1] 徐志文[1] 陈杨[1] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014

出　　处：《西南农业学报》2007年第4期800-806,共7页Southwest China Journal of Agricultural Sciences

基　　金：教育部长江学者和创新团队发展计划项目(IRT0555);四川省科技厅基础项目(03JY029-40)

摘　　要：克隆测定12条PRV不同毒株的gD全序列连同Genebank中登录的9条gD基因全序列共21条基因序列，使用生物软件对它们的基因序列的同源性、突变区域的定位、遗传进化关系、氨基酸序列的同源性、蛋白质亲水性、抗原表位分析、三级结构预测等生物信息学的内容进行预测和分析。结果表明：PRV—gD基因的开放阅读框的核苷酸长度在1197～1215nt之间，氨基酸长度在399—405个之间，核酸同源性在97．3％-100％之间，氨基酸的同源性在89．8％～98．8％之间，在核酸820—837位有个高变重复区。在遗传进化关系上将我国PRV流行分为四川、华北、东南三个区域。该结果说明PRV—gD基因具有很高的保守性。12 sequenced gD genes of different PRV strains, as well as 9 gD genes which were downloaded from GenBank were analyzed by bioirfformafics software, the analysis and prediction of these genes including nucleotide homology, location of mutation area, phylogenetic tree, amino acids homology, pn3tein hydrophilicity, epitope and three-dimensional structure. The results showed that the ORF length of PRV-gD gene is 1197 - 1215nt, amino acids length is 399 -405 ,nucleotide homology is 97.3% - 100% ,amino acids homology is 89.8% -98.8% ,a hypermutation replicated plot is located in 820-837nt. According to the phylogenetic tree, PRV prevalence in China can be divided into three regions, which are Sichuan province, Northern China and Southeast China. These results suggested that the gD gene of PRV is highly conserved.

关 键 词：伪狂犬病病毒 GD基因 克隆 序列分析 生物信息学 

分 类 号：S858.28[农业科学—临床兽医学]