应用微波前处理-热水浸提技术提取龙眼多糖  

Hot water extraction of longan polysaccharide assisted by microwave pretreatment

作　　者：杨翠娴[1] 李清彪[1] 凌雪萍[1] 邵文尧[1] 徐慧[1] 

机构地区：[1]厦门大学化学化工学院化学工程与生物工程系化学生物学福建省重点实验室,福建厦门361005

出　　处：《化工学报》2007年第8期2004-2009,共6页CIESC Journal

基　　金：厦门市翔安区科技局资助项目

摘　　要：龙眼多糖具有抗氧化、抗衰老等多种生物活性,但对龙眼多糖提取工艺和其化学结构方面的研究文献报道较少。采用微波前处理-热水浸提新工艺提取龙眼多糖,单因素考察及正交实验结果表明,在所考察实验范围内,龙眼多糖的最佳提取工艺条件为:微波前处理功率700W,处理时间60s,热水浸提料液比1∶15,浸提温度100℃,浸提时间7h,搅拌速率240r.min-1。在此条件下,龙眼多糖收率可达9.00mg.(g龙眼)-1(干重)。紫外和红外光谱分析结果显示,所获得的龙眼多糖是具乙酰氨基结构的β型吡喃酸性杂多糖。Longan polysaccharide （LPS） has been discovered to have high anti-oxidation and anti-senility activity, while only a few studies about its extraction and chemical structure analysis have been reported. In this paper, longan pulp was pretreated by microwave and then LPS was extracted with hot water. The results of single-factor examination and orthogonal experiments showed that the optimal process parameters for this method were： microwave pretreatment with power 700 W for 60 s, followed by hot water extraction with solid （g） liquid （ml） ratio of ] ： 15 at ]00℃ for 7 h with stirring speed of 240 r· min^-1. The optimal yield of 9.00 mg·g^-1 （dried longan biomass weight） was achieved under these parameters. Ultraviolet spectrum and FTIR analysis showed that LPS was a β type acidic heterosaccharide with pyran and acetyl amino group.

关 键 词：龙眼 多糖 微波 提取 

分 类 号：R284.2[医药卫生—中药学]