世界范围内A组轮状病毒G1型毒株VP7基因及VP4基因高变区的分析  

Divergence of VP7 gene and VP4 gene variable regions of G1 rotaviruses isolated from different geographic locations around the world

作　　者：金奇[1,2] 曾力宇[1,2] Jing.H RogerI.Glas JonR.Gentsch 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 [2]美国疾病控制中心

出　　处：《中华实验和临床病毒学杂志》1997年第1期3-7,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：Ａ组轮状病毒的ＶＰ４和ＶＰ７多肽是刺激机体产生中和抗体的主要保护性抗原。我们总结了世界多个地区和国家Ｇ１型轮状病毒流行株ＶＰ４和ＶＰ７基因的分子流行病学调查结果，并根据ＶＰ７多肽序列绘制了分子系统分类树。结果表明，所有流行株ＶＰ４基因的高变区具有相似的氨基酸组成，而ＶＰ７多肽疏水区，特别是已知的抗原决定簇ＶＲ３，ＶＲ４及ＶＲ６的氨基酸组成有规律性变异，提示这些毒株间在抗原性上有微小差异。分子系统分类树将所有毒株分为三个与氨基酸组成相对应的亚组，各个亚组的成员构成反映出了世界范围内轮状病毒流行株的分布具有较明显的地域性。Group A rotaviruses are the most important etiologic agents of severe gastroenteritis in children, and as a result, the development of an effective vaccine against severe rotavirus diarrhea is a top public health priority. Previous studies indicated that G1 rotavirus are much more common in most countries around the world and development of G1-specific vaccine may be important in protection against human illness. In this report, we analyzed the VP7 gene and VP4 gene variable regions of 36 G1 rotavirus community strains collected from different geographic locations worldwide. Comparative analysis of the VP7 sequences showed that most of the amino acid substitutions of global strains occurred in and around previously identified epitope regions VR3, VR4, and VR6, suggesting that there are slight antigenic differences among these strains. Growtree phylogram using the VP7 amino acid sequences as an index grouped all strains into three clusters. The VP7 sequence of each cluster possesses distinctive amino acid residues which are peculiar to most members of the same group at a most positions. These data will be essential to achieve a much better understanding of molecular epidemiologic characteristics of G1 rotavirus around the world.

关 键 词：轮状病毒属 流行病学 基因 病毒 分子流行病学 

分 类 号：R373.2[医药卫生—病原生物学]