丙型肝炎病毒在人T淋巴细胞中的体外感染  

In Vitro infection of the human T-cell line with the hepatitis C virus

作　　者：叶进[1,2] T.Goeser 

机构地区：[1]同济医科大学协和医院传染科 [2]德国海德堡大学内科医院消化内科

出　　处：《中华实验和临床病毒学杂志》1997年第1期41-43,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：用丙型肝炎病毒（ＨＣＶ）ＲＮＡ阳性的血清感染人类Ｔ淋巴细胞（Ｍｏｌｔ－４），１２小时后继续孵化１～２８天，将感染后的产物用聚合酶链反应（ＰＣＲ）测定，于第１、２、３天和第８天发现ＨＣＶ－ＲＮＡ的正链，第２和第８天发现ＨＣＶ－ＲＮＡ的负链。A human T-cell line (Molt-4) was infected with an hepatitis C virus RNA (HCV-RNA) positive serum for 12 hours and incubated continously for 1-28 days. A productive infection was demonstrated by polymerase chain reaction amplification. Viral plus-stranded RNA was detectable in Molt-4 on the 1st, 2nd, 3rd day and the 8th day post infection. Viral minus-stranded RNA was detectable on the 2nd day and 8th day post infection.

关 键 词：丙型肝炎 T淋巴细胞 感染 丙型肝炎病毒 

分 类 号：R512.63[医药卫生—内科学] R373.21[医药卫生—临床医学]