电融合技术与半固体培养技术结合制备抗人肠道腺病毒单克隆抗体  

Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture

作　　者：张云[1] 陈力真[1] 王树蕙[1] 郭彩云[1] 貌盼勇 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《中华实验和临床病毒学杂志》1997年第1期69-71,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：将１×１０７个经肠道腺病毒（Ａｄ４０／４１）免疫的小鼠脾细胞与３×１０６个骨髓瘤细胞进行电融合，再置ＩＭＤＭ甲基纤维素半固体选择培养基中进行选择培养，一次性得到１８个杂交瘤细胞克隆，并从中筛查出７株高阳性抗体分泌克隆。从而成功地运用电融合与半固体培养相结合的方法以少量的材料制得了抗肠道腺病毒单克隆抗体。Ten million splenocytes from the enteric adenovirus (Ad40/41) immunized mice were fused with 3×10 6 myeloma cells by electrofusion. Then, the cells were selectively cultured in methyl cellulose semi-solid medium. Eighteen clones of hybridoma were directely picked out from the culture plates and 7 of them could secret high level specific antibodies to enteric adenovirus. So far, the anti-Ad40/41 virus monoclonal antibodies were successfully generated by the protocal of electrofusion accompanied with selective culture in methyl cellulose semi-solid medium.

关 键 词：电融合 半固体培养 肠道腺病毒 单克隆抗体 制备 

分 类 号：R392-33[医药卫生—免疫学]