外源性皮质醇对异育银鲫TSH-βmRNA表达和血清甲状腺激素水平的影响  

Effects of exogenous cortisol on TSH-β subunit mRNA level and serum thyroid hormone levels of Carassius auratus gibelio

作　　者：曲宪成[1] 杨艳红[1] 刘颖[1] 周正峰[1] 崔严慧[1] 

机构地区：[1]上海水产大学生命科学与技术学院,上海200090

出　　处：《上海水产大学学报》2007年第4期305-309,共5页Journal of Shanghai Fisheries University

基　　金：上海市重点学科建设项目资助(Y1101);上海市教委发展基金资助(科04-135);回国留学人员科研启动基金(科01-113)

摘　　要：运用Sybr Green I荧光定量RT-PCR方法和放射免疫方法(RIA)分析了外源性皮质醇(cortisol)对异育银鲫促甲状腺激素β亚基(TSH-β)mRNA表达和血清甲状腺激素水平的影响。实验设计为10周,实验组每两周注射一次0.2 mg/kg体重的cortisol(用57%酒精溶解),对照组注射同剂量的57%酒精。实验期间没有投喂饲料。在本实验条件下,研究结果显示:注射3次外源性cortisol后,异育银鲫TSH-βmRNA表达量与对照组相比略有下降;注射5次后,与对照组相比显著降低(P<0.05)。另一方面,RIA结果显示:注射3次外源性cortisol后,实验组与对照组相比,血清甲状腺激素T3和T4含量虽略有升高,但无显著差异;而注射5次后显著升高(P<0.05)。以上结果提示:外源性cortisol能够影响异育银鲫脑下垂体-甲状腺轴。In this study, Sybr Green Ⅰ fluorescence quantitative reverse transcription polymerase chain reaction and radioimmunoassay （RIA） methods were used to investigate the effects of exogenous cortisol on thyroidstimulating hormone βsubunit （TSH-β） mRNA and serum thyroid hormone levels of Carctssius auratus gibelio. The study was carried out in 10 weeks, during which cortisol was injected every two weeks for experiment groups at a dose of 0.2 mg per kilogram body weight （dissolved in 57% ethanol） , while the same dose of 57% ethanol was injected for control groups. The fish were not fed throughout the experimental period. The results showed that after 3 times of cortisol injection, TSH-β mRNA level decreased slightly compared with control group, but did not show significant difference; after 5 times of cortisol injection, TSH-β mRNA level decreased significantly （ P 〈 0.05 ）. On the other hand, the serum thyroid hormones RIA results showed that after 3 times of cortisol injection, T3 and T4 concentrations increased slightly; after 5 times of cortisol injection, T3 and T4 concentrations increased significantly （P 〈0.05）. From these results we concluded that exogenous cortisol could influence pituitary-thyroid gland axis function of Carctssius auratus gibelio.

关 键 词：异育银鲫 皮质醇 SYBR Green Ⅰ荧光定量RT-PCR 促甲状腺激素β亚基 放射免疫方法 T3 T4 

分 类 号：S917[农业科学—水产科学]