RP-HPLC法测定保康北柴胡中柴胡皂苷a、d的含量  

Determination of Saikosaponin a,d in Bupleurum by RP-HPLC

作　　者：熊梦晓[1] 黄必胜[1] 白杨[1] 范文乾[1] 胡继鹰[1] 

机构地区：[1]湖北中医学院,湖北武汉430061

出　　处：《现代中药研究与实践》2007年第2期42-44,共3页Research and Practice on Chinese Medicines

基　　金：湖北省自然科学基金(2005ABA187)

摘　　要：目的 采用RP-HPLC法测定保康北柴胡中柴胡皂苷a、d的含量.方法 色谱柱：BonChrom C18柱（4.6 mm×200 mm,5 μm）,流动相：乙腈-水（40∶60）,流速：1.0 mL·min-1,检测波长：204 nm.结果 柴胡皂苷a：Y=7.131 4X＋2.0118,r=0.999 7,线性范围：2.136～21.360 μg;柴胡皂苷d：Y=9.032 3X＋3.739 2,r=0.999 0,线性范围：2.656～26.560 μg.平均回收率：柴胡皂苷a 99.3%（RSD=0.73%）,柴胡皂苷d99.4%（RSD=0.82%）.结论 本法简便快捷,结果准确,重复性好,GAP基地的柴胡皂苷含量较高.Objective A HPLC method was established for the determination of Saikosaponin a and Saikosaponin d in Bupleurum. Methods A BonChrom C lscolumn was used with acetonitrile-water（40： 60） as the mobile phase and in a flow rate of 1.0 mL · min ^- 1. The detecting wavelength was at 204nm. Results There was a good linearity in the range of 2. 136 -21. 360 μg of Saikosaponin a （ r = 0.999 7 ） and 2. 656 - 26. 560μg of Saikosaponin d （ r = 0. 999 0 ） , The average recovery was 99.3 % and 99.4% , respectively. Conclusion The method was simple, rapid, accurate and reproducible. It has also the higher Saikosaponin content of Bupleurum chinense in GAP Base.

关 键 词：柴胡 柴胡皂苷 RP-HPLC 

分 类 号：R284.1[医药卫生—中药学]