胰岛素降解酶基因多态性与多囊卵巢综合征相关性的研究  

Single nucleotide polymorphism of insulin degrading enzyme gene correlation with polycystic ovary syndrome

作　　者：王克华[1] 陈子江[1] 石玉华[1] 王来城[2] 游力[1] 

机构地区：[1]山东大学山东省立医院生殖中心 [2]山东大学山东省立医院 中心实验室,济南250021

出　　处：《现代妇产科进展》2007年第7期521-524,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金(No:30470703)

摘　　要：目的:探讨胰岛素降解酶基因(IDE)多态性与多囊卵巢综合征(PCOS)发病的关系。方法:用聚合酶链反应-限制性片段多态性技术,检测315例PCOS患者(PCOS组)和327例健康妇女(对照组)的IDE基因多态性。结果:IDE基因rs1887922位点基因型分布及等位基因频率在PCOS组总体以及各亚组(胰岛素抵抗组及非胰岛素抵抗组),与健康对照组之间比较均无显著差异。而IDE基因rs2209972位点基因型分布虽然在P- COS组总体及非胰岛素抵抗亚组与健康对照组之间比较无显著差异;但PCOS组总体相应位点的C等位基因频率高于对照组,差异有显著性(P<0.05),PCOS胰岛素抵抗亚组相应位点的基因型频率及等位基因频率均高于对照组,差异有统计学意义(P<0.05)。rs2209972位点不同基因型PCOS患者空腹胰岛素水平及HOMA-IR存在显著差异(P<0.05)。结论:胰岛素降解酶基因多态性可能与PCOS患者胰岛素抵抗相关。Objective ：To investigate the correlation between single nucleotide polymorphism of insulin degrading enzyme （ IDE ） gene and polycystic ovary syndrome （ PCOS ）. Methods：Three hundred and fifteen patients with PCOS（PCOS group）and 327 normal women（control group）were included. Genotyping of two single nucleotide polymorphism of IDE gene was performed using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） Results：The distribution of genotype of rs1887922 in IDE gene was similar in PCOS patients,PCOS-insulin resistance （PCOS-IR） group, PCOS-non-insulin resistance （PCOS-NIR） group and controls. The distribution of genotype of rs2209972 in IDE gene was similar in PCOS group, PCOS-NIR group and controls, but the C allele frequency of rs2209972 in IDE gene was significantly higher in PCOS patients than that in controls. The distribution of genotype and the C allele frequency of rs2209972 in PCOS-IR group were significantly than that in controls. Both the PCOS women with CC and CT genotype of rs2209972 had statistically significantly higher fasting insulin level and HOMA-IR than that of the PCOS women with TT genotype. Conclusion ：The single nucleotide polymorphism of IDE gene may play an important role in the occurrence of insulin resistance in patients with PCOS.

关 键 词：多囊卵巢综合征 胰岛素降解酶 胰岛素抗药性 多态性 单核苷酸 

分 类 号：R711.7[医药卫生—妇产科学]