籼稻成熟胚遗传转化体系影响因素的优化  

Optimization of Transformation Mediated by Agrobacterium tumefaciens in Indica Rice

作　　者：曾晓珊[1] 戴良英[1] 刘雄伦[1] 吴俊[1] 宁约瑟[1] 高佳[1] 王国梁[1] 

机构地区：[1]湖南农业大学水稻基因组学实验室

出　　处：《江西农业学报》2007年第8期1-3,6,共4页Acta Agriculturae Jiangxi

基　　金：国家自然科学基金(30571063;30470990);湖南农业大学"芙蓉学者"计划项目;湖南省教育厅科学研究项目(04A024;05B028);湖南省杰出青年基金(06JJ10006)

摘　　要：以5个籼稻品种为研究材料,对影响籼稻成熟胚愈伤组织培养、愈伤组织与根癌农杆菌共培养的部分因素进行了研究.结果表明：适当提高硝态氮和铵态氮比例、1.0～2.5mg/L的2,4-D浓度有利于籼稻品种成熟胚愈伤组织的诱导;愈伤组织继代12d左右共培养效果较好;愈伤组织与农杆菌液共培养3d能获得50%以上的转化率.Five indica rice cultivars were investigated to study several factors influential to transformation frequency. Appropriate higher ratio of nitrate nitrogen to ammonium nitrogen, 1.0 - 2.5mg,/L 2,4 - D were beneficial to indica flee callus inducement. The results of subculture time investigation showed that twelve days＇ culture conduced to callus activity. Advanced research results showed that three days＇ co - culture of callus and A. tumefacien was beneficial to target gene＇ s transformation.

关 键 词：籼稻 成熟胚 愈伤组织 组织培养 农杆菌介导 

分 类 号：S511.21[农业科学—作物学]