福建三尖杉活性成分研究 Ⅰ.三尖杉酯碱的提取与光谱鉴定  

Study on Active Compounds of Cephalotaxus in Fujian Province Ⅰ.Extraction and Spectral Identification of Harringtonine

作　　者：郭文杰[1] 方少忠[1] 蔡宣梅[1] 林真[1] 鲁雪华[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《江西农业学报》2007年第8期108-110,共3页Acta Agriculturae Jiangxi

基　　金：福建省科技厅项目(2002Y024-2)

摘　　要：采用乙醇回流提取法得到三尖杉药用成分的粗提物,然后利用有机物梯度萃取、逆流分布法分离,结合氧化铝柱和薄层层析法对粗提物进行分离提纯,最后利用四大光谱:质谱(MS)、红外光谱(IR)、紫外光谱(UV)和核磁共振光谱(NMR)对提纯的化合物进行结构鉴定,确定三尖杉体内含有抗癌活性成分三尖杉酯碱。The crude extract of active compounds of Cephalotaxus was gained with alcohol circumfluence distillation, then purified through gradient extraction of organic substance, separation of countereurrent distributing, alumina -pillar and Thin Layer Chromatography （TLC）. The configuration of active compounds was identified by four kinds of spectrums ： Mass spectrum（ MS）, Infrared spectrum（IR）, Ultraviolet spectrum（UV） and Nucleus magnetism resonance spectrum（NMR）. It was confirmed that Cephalotaxus contained Harringtonine which was an anti - cancer active compound.

关 键 词：三尖杉 提取 三尖杉酯碱 光谱鉴定 

分 类 号：S791.48[农业科学—林木遗传育种]