小剂量FK778预防大鼠移植肾的慢性排斥反应  

Effect of low-dose FK778 on the progression of chronic renal ailograft rejection in a rat model

作　　者：魏立新[1] 舒贵扬[1] 邹和群[2] 余学清[3] 

机构地区：[1]福建医科大学福建省立临床医学院肾内科,福州350001 [2]中山大学附属第五医院肾内科 [3]中山大学附属第一医院肾内科

出　　处：《中华器官移植杂志》2007年第8期488-491,共4页Chinese Journal of Organ Transplantation

摘　　要：目的研究小剂量FK778对大鼠移植肾慢性排斥反应的预防作用。方法应用显微外科技术制作大鼠移植肾慢性排斥反应模型;将肾移植后的大鼠分为两组。治疗组:将FK778溶解在羧甲基纤维素溶液中,行灌胃治疗,自术后第1天开始,每天1次,剂量为5mg·kg^(-1)·d^(-1),治疗至肾移植后24周。对照组:用单纯羧甲基纤维素溶液对大鼠行灌胃治疗,每天1次,直至移植后24周。移植后每4周测定1次24h尿蛋白含量,第24周时处死大鼠,对移植肾行组织学、免疫组织化学及实时定量逆转录聚合酶链(RT-PCR)检测。结果治疗组大鼠24h尿蛋白含量、肾组织病理损害程度、淋巴细胞和单核/巨噬细胞浸润程度与对照组比较均显著减低,肾组织生长因子TGF-β、PDGF-B基因的表达也显著减少。结论小剂量FK778能预防大鼠移植肾慢性排斥反应。Objective To study the effect of low-dose FK778 in preventing chronic renal allograft rejection in rats. Methods The rat model of chronic renal allograft rejection was established by using micro-surgery technique. The recipients were divided into two groups. The recipients in the study group were treated with FK778 at a dose of 5 mg·kg^-1 · d^-1 dissolved in carboxymethylcellulose by means of gavage and the controls were treated with carboxymethylcellulose. Urinary protein concentrations were measured every 4 weeks for 24 weeks. On 24th week after operation, the rats were killed and the kidney grafts were taken out for histological and immunohistological examinations as well as quantitative real-time RT-PCR analysis. Results After 24 weeks of treatment, proteinuria, the severity of chronic rejection, glomerulosclerosicytes and monocytes/macrophages in the study group were significantly milder than in control group. And the expression of TGF-13 rnRNA and PDGF-B rnRNA was significantly reduced in the study group as compared with that in the control group. Conclusion Low-dose of FK778 might prevent the rats from chronic renal allograft rejection.

关 键 词：免疫抑制剂 肾移植 移植物排斥 

分 类 号：R699.2[医药卫生—泌尿科学]