骨髓基质细胞移植促进大鼠局灶性脑损伤血管生成的实验研究  

The experimental study on promoting vascularization by bone marrow stromal cells transplantation after local brain injury in rats

作　　者：张鹏飞[1] 张亚卓[1] 李庆国[1] 孙梅珍[1] 王红云[1] 何乐[1] 

机构地区：[1]北京市神经外科研究所,首都医科大学附属北京天坛医院,100050

出　　处：《中华神经外科杂志》2007年第8期633-635,共3页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金资助(30271323)

摘　　要：目的研究骨髓基质细胞(BMSCs)移植对大鼠局灶性脑损伤血管生成的影响,探讨BMSCs移植修复大鼠脑损伤的机制。方法制备大鼠局灶性脑损伤动物模型,进行BMSCs移植,通过免疫组织化学、电镜等观察移植局部内皮细胞及微循环的变化。结果BMSCs移植后,局部凝血因子FⅧ染色阳性细胞数增加,染色加深,出现血管样结构;局部微血管内膜较光滑,内皮细胞间可见紧密连接,细胞核形态较规则,基底膜较完整,微血管管腔受压缓解。结论BMSCs移植促进损伤区周围内皮细胞增殖和新生血管形成。损伤区局部微循环得以改善进而修复脑损伤。Objective To observe the effects of bone marrow stromal cells transplantation on vascularization after local brain injury in rats, and to investigate the mechanism of repairing by BMSC transplantation. Methods The animal model local brain injury in rat was established. The hBMSCs were transplanted into the local brain. The immunohistochemistry and reactance were used to observe endothelial cell and microcirculation of local brain. Results The hBMSCs were transplanted into the animal model, the quantities and the expression of the F ⅧR Ag positive cells increased and the microvascular structure was formed; the inside wall of the microvessel was smooth, the union between the cells was firm; nucleuses were regularly organized, basement membranes were continued, and the vascular compression was relief. Conclusions Local vascular endothelial cells were promoted to proliferate when hBMSCs were transplanted and the microvessel was formed increaselly, Microcirculation of local brain was improved, and brain injury was repaired.

关 键 词：骨髓基质细胞 脑损伤 血管生成 

分 类 号：R651.15[医药卫生—外科学]