马尾松树皮提取物抗肿瘤作用的研究  

Study on Anti-tumor Activity of Extract from Masson′s Pine Bark

作　　者：郑光耀[1] 李若达 王成章[1] 谢衡 叶建中[1] 高彩霞[1] 

机构地区：[1]中国林业科学研究院林产化学工业研究所国家林业局林产化学工程重点开放性实验室,江苏南京210042 [2]广东嵩珍营养源研究所,广东乐昌512229

出　　处：《林产化学与工业》2007年第4期16-20,共5页Chemistry and Industry of Forest Products

基　　金：中国林业科学研究院科学技术发展基金资助项目(2005-M-08);广东省工业攻关计划资助项目(2004B10401033)

摘　　要：研究马尾松树皮提取物(MPBE)体外和体内的抗肿瘤作用。采用四甲基偶氮唑蓝比色(MTT)法观察MPBE对人结肠腺癌细胞株LoVo的体外抑制作用,采用小鼠移植性肿瘤模型考察MPBE对S180荷瘤小鼠的抑瘤作用和艾氏腹水癌(EAC)小鼠的生命延长作用。结果表明:MPBE对Lo-Vo细胞的生长有较强的抑制作用,在质量浓度为500 mg/L时,MPBE对LoVo细胞作用24、48和72 h时的抑制率分别为19.83%、33.06%和58.15%;MPBE对小鼠S180肉瘤生长表现出明显的抑制作用,MPBE 50、100和200 mg/kg 3个剂量的抑瘤率分别为30.3%、37.8%和42.3%,MPBE达到200 mg/kg剂量时,反映免疫功能的胸腺指数和脾指数也明显增加;但MPBE对EAC腹水癌小鼠的生存时间没有明显的延长作用。To study the anti-tumor activity of Masson＇s pine bark extract （MPBE） in vitro and in vivo, MTT assay was used to observe the growth inhibitory effect of MPBE on human colonic cancer cell line VoLo in vitro. The model of transplanted tumor in mice was used to observe the inhibiting effect on S180 sarcoma in mice, and the survival time of mice bearing Ehrlich ascites carcinoma （EAC） , by calculating thymus and spleen indexes in mice bearing S180 sarcoma. Results showed： MPBE could inhibit the growth of LoVo cells in vitro, the inhibitory rates of MPBE at the concentration of 500 mg/L were 19.83 %, 33.06 % and 58.15 % after incubation for 24, 48 and 72 h respectively; MPBE could significantly inhibit the growth of S180 sarcoma in mice, the inhibitory rates of MPBE were 30.2 %, 37.8 % and 42.3 % at the dosags of 50, 100 and 200 mg/kg respectively. MPBE at the dose of 200 mg/kg could also increase the thymus and spleen indexes in mice bearing S180 sarcoma significantly. However, MPBE could not prolong the survival time of mice bearing Ehrlich ascites carcinoma. MPBE has obvious inhibitory effect on the growth of LoVo cells in vitro and S180 sarcoma in mice, it could be a potential chemopreventive or chemotherapeutic agent of cancer.

关 键 词：马尾松树皮提取物 LOVO细胞 S180荷瘤小鼠 抗肿瘤活性 

分 类 号：TQ424.19[化学工程] O532.23[理学—等离子体物理]