白桦不同部位及种源间白桦酯醇含量的差异分析  

Research on Difference of Betulin Content in Different Parts and Provenances of Birch(Betula platyphylla Suk.)

作　　者：范桂枝[1] 詹亚光[1] 王博[1] 邱磊[1] 刘桂丰[2] 王会仁[2] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]东北林业大学林木遗传育种与生物技术教育部重点实验室,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2007年第4期103-106,共4页Chemistry and Industry of Forest Products

基　　金：黑龙江省科技攻关项目(GA06B301-3-3);东北林业大学引进人才项目(010-602023)

摘　　要：以白桦为研究材料，在白桦酯醇提取方法的选择下，采用高效液相色谱仪（HPLC）分析了白桦不同部位和种源间白桦酯醇含量的变化。结果表明：索氏提取和超声波醇提的白桦酯醇含量较高，分别为240．25和239．57mg／g，而回流法和氯仿浸提的白桦酯醇含量稍低，为207.88和194．09mg／g。白桦酯醇在白桦不同部位的含量分布差异显著，依次分别为：外皮〉枝皮〉内皮〉芽〉叶〉根〉花粉；13种8年生白桦种源树皮内白桦酯醇的含量为132．45—257．11mg／g，且种源间差异呈极显著水平。进一步分析可得，同龄树种的生长状况对白桦酯醇的含量影响不显著；而白桦酯醇的含量不仅受自身种源及遗传背景的影响，同时也受到其所处地理位置和气候因素的影响，即属于基因与环境互作的表现性状。To understand the difference of the betulin content in different parts and provenances in Betula platyphylla Suk. , the methods to extract betulin were selected, and an accurate HPLC method to detect betulin content was used. The results were as follows ： Betulin contents of the same birch bark through 4 extraction methods, namely Soxhlet extraction, supersonic wave, extraction refluxing and chloroform extraction were 240.25,239.57,207.88 and 194.09 mg/g respectively; The contents of betulin in different parts of birch were outer bark 〉 branch bark 〉 inner bark 〉 bud 〉 leaf 〉 root 〉 pollen ; Betulin contents in the bark of eight-year old birches of 13 provenances were detected, and their range was from 132.45 to 257.11 mg/g. Analyzing the relations among betulin content and growth status, geographical location and climatic factors, it was found that the effect of growth status on betulin content was not significant, and betulin content was not only affected by self-provenance and heredity background but also by geographical location and climatic factor.

关 键 词：白桦 白桦酯醇 提取方法 不同部位 种源 

分 类 号：TQ91[化学工程]