大孔树脂ADS-7分离纯化多穗柯黄酮工艺研究  

Separation and Purification of Flavonoid from Lithocarpus polystachyus(Wall.) Rehd. with Macro-porous Resin ADS-7

作　　者：董华强[1] 宁正祥[1] 李琳[1] 于立静[1] 林丽超[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510641 [2]佛山科学技术学院食品科学系,广东佛山528231

出　　处：《林产化学与工业》2007年第4期120-122,共3页Chemistry and Industry of Forest Products

基　　金：广东省自然科学基金资助项目(20020842);广东省科技计划资助项目(2003C20506)

摘　　要：采用大孔树脂ADS-7分离纯化多穗柯黄酮粗提物(CE),对主要工艺条件进行了研究。得出最佳工艺条件为:将CE先配成10%(体积分数)CE溶液,10%CE溶液体积与树脂质量比值(mL∶g)略大于3、在中性pH值下静态吸附2 h,再以3倍装柱体积(1 500 mL)的80%(体积分数)乙醇、每小时1倍装柱体积流速进行动态洗脱。该工艺条件得到的纯化产物中黄酮的质量分数由粗提物中的40%提高到纯化产物的88%,得率为91%。The flavonoid was separated and purified from the crude extract of Lithocarpus polystachyus（ Wall. ） Rehd. leaves with macro-porous resin （MPR） ADS-7, and the technology conditions were investigated in this experiment. The results showed that the optimum condition of the technology was that the ratio of the crude extract solution/resin was a little bigger than 3, static adsorption time was 2 h at neutral pH value, 1 500 mL 80 % （volume fraction ） ethanol as eluant, and eluation speed was 500 mL/h. By this technology the flavonoid content was increased from 40 % of the crude extract to 88 % of the purified product and the yield was 91%.

关 键 词：大孔树脂 多穗柯 黄酮 纯化 

分 类 号：TQ91[化学工程]