纳秒级陡脉冲对人卵巢癌细胞SKOV_3生长抑制及诱导凋亡作用  

Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV_3

作　　者：吴晓娟[1] 姚珍薇[1] 唐均英[1] 姚陈果[2] 孙才新[2] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016 [2]重庆大学电气工程学院高电压与电工新技术教育部重点实验室,重庆400044

出　　处：《第三军医大学学报》2007年第17期1670-1672,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金重点项目(50637020);教育部博士点基金(20050611020);重庆市科委科技项目(2005AA5008-3;2005BB5251)~~

摘　　要：目的探讨纳秒级陡脉冲对人卵巢癌细胞的生长抑制和凋亡诱导作用。方法采用人卵巢癌细胞SKOV3,应用MTT比色法,倒置显微镜,荧光显微镜及Annexin-FITC技术等方法进行检测和观察。结果中(8.5kV/cm)、高(10kV/cm)场强处理组对SKOV3细胞活性具有明显抑制作用,呈剂量依赖性,在处理后2～4h内呈时间依赖性;倒置显微镜和荧光显微镜下观察到典型的凋亡细胞形态;流式细胞仪示,中、高场强处理组早期凋亡率分别为(22.21±2.71)%、(57.30±6.51)%,与对照组(3.04±0.44)%比较均有显著性差异。结论一定范围内的纳秒级陡脉冲能明显抑制SKOV3细胞增殖,亦诱导SKOV3细胞凋亡。Objective To investigate the effect of nanosecond steep pulse on growth and apoptosis of human ovarian carcinoma cells in vitro. Methods MTF assay, invert microscopy, fluorescence microscopy and annexinV-FITC were used to identify the inhibitory effect of nanosecond steep pulse on cell proliferation and the induction of apoptosis in human ovarian carcinoma cell Line SKOV3. Results Nanosecond steep pulse with 10 and 12.5 kV/cm significantly inhibited the viability of SKOV3 cells in a dose-dependent manner, and in a timedependent manner within 2 to 4 h of nanosecond steep pulse treatment. The typical changes of SKOV3 cell apoptosis were observed by invert microscopy and fluorescence microscopy. Early apoptotic rate of SKOV3 treated with 10,12.5 kV/cm nanosecond steep pulse was （22.21 ± 2.71 ）% and （57.30 ± 6.51 ）%, respectively, significantly higher than those of the control group （ 3.04 ± 0.44 ） % （ P 〈 0.05 ）. Conclusion Nanosecond steep pulse in certain range can inhibit the growth of SKOV3 cells as well as induce the apoptosis.

关 键 词：陡脉冲 卵巢肿瘤 SKOV3细胞株 生长抑制 凋亡 

分 类 号：R454[医药卫生—治疗学] R73-36[医药卫生—临床医学]