5-羟色胺对大鼠膈下迷走神经传入放电的影响及机制  

Effect of serotonin on subdiaphragmatic vagus nerve afferent discharge and its mechanism in rats

作　　者：隋红[1] 陈卫[1] 陆杰[1] 

机构地区：[1]重庆医科大学基础医学院生理学教研室,重庆400016

出　　处：《第三军医大学学报》2007年第17期1706-1708,共3页Journal of Third Military Medical University

摘　　要：目的探讨大鼠外周静脉注射5-羟色胺以及促胰液素(secretin,SEC)对膈下迷走神经传入冲动的影响,以及5-羟色胺对膈下迷走神经传入冲动影响的机制。方法采用电生理学方法记录膈下迷走神经传入自发放电为观察指标,观察外周静脉注射不同剂量的5-HT(3、10、30μg/kg)、5-HT3受体的拮抗剂格拉司琼(1mg/kg)以及5-HT+SEC对其的影响。结果5-HT对膈下迷走神经传入自发放电具有兴奋作用,给予5-HT3受体拮抗剂后神经放电的效应被抑制,大剂量5-羟色胺+促胰液素可增强由单独的大剂量5-HT所致的迷走神经传入放电的兴奋作用。结论5-HT可能是作用于与迷走传入相关的5-HT3受体兴奋了膈下迷走传入神经自发放电;促胰液素可以增强5-HT对迷走神经传入自发放电的兴奋作用。Objective To investigate the effect and mechanism of serotonin （5-HT） and secretin（ SEC ） on afferent discharge of vagus in rats. Methods Spontaneous afferent discharge of subdiaphragmatic vagus nerve was recorded in urethane anesthetized rats. The effects of 5-HT, 5-HT3 receptor antagonist and 5-HT ＋ SEC on the discharge of subdiaphragmatic vagus nerve were investigated by intravenous injection of different dosage of5-HT（3, 10, 30 μg/kg）, Granisetron （1 mg/kg） and 5-HT ＋ SEC. Results Intravenous 5-HT caused obvious exciting effect on spontaneous afferent discharge of subdiaphragmatic vagus nerve. The 5-HT3 receptor antagonist significantly inhibited the effects of 5-HT. 5-HT ＋ SEC could augment the effect of 5-HT on spontaneous discharge. Conclusion The exciting effect of 5-HT on afferent discharge of subdiaphragmatic vagus nerve is possibly mediated by 5-HT3 receptor related to vagus nerve afference. Secretin can augment the exciting effect of 5-HT on spontaneous discharge of vagus afferent nerve.

关 键 词：5-羟色胺 膈下迷走神经 格拉司琼 促胰液素 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R338[医药卫生—基础医学]